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Journals / Turkish Journal of Veterinary and Animal Sciences / 2011 / Cilt: 35 - Sayı: 5

Chronic continuous perfusion of the hibernation triggering opioid DADLE did not cause any major physiological alterations in rats

İbrahim AKYAZI
Ülker ÇÖTELİOĞLU
Pages
325–330
Publication Date
2011-05-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

While thrombin time was significantly lengthened in the DADLE treated group, there were no statistically significant differences in the other investigated parameters between the experimental group and the control group.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:[D-Ala2, D-Leu5] enkephalin (DADLE), a delta opioid receptor agonist, can induce mammalian hibernation and has hibernation-like effects in non-hibernating species.
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Key Findings & Data:It has also been demonstrated that DADLE has protective efficacy on various tissues such as nervous, cardiac, and hepatic tissues.
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Conclusion & Impact:While thrombin time was significantly lengthened in the DADLE treated group, there were no statistically significant differences in the other investigated parameters between the experimental group and the control group.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Key words: DADLE · opioid · hibernation

Abstract

[D-Ala2, D-Leu5] enkephalin (DADLE), a delta opioid receptor agonist, can induce mammalian hibernation and has hibernation-like effects in non-hibernating species. It has also been demonstrated that DADLE has protective efficacy on various tissues such as nervous, cardiac, and hepatic tissues. Despite substantial research conducted in the last decade about the tissue protective efficacy of DADLE, detailed underlying mechanisms have not been fully elucidated. In the present study we investigated in a rat model whether continuous perfusion of DADLE can induce potentially tissue protective alterations in physiological parameters. In the study 48 young male Wistar rats were used. They were divided into control and experimental groups, each group consisting of 24 animals. DADLE was perfused to experimental group rats continuously for 28 days using subcutaneously implanted osmotic mini-pumps. We investigated its effects on body temperature, antioxidant protection [total antioxidant capacity (TAC), ascorbic acid (AA), and malondialdehyde (MDA) levels], white blood cell (WBC) count, and coagulation parameters [activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT)]. While thrombin time was significantly lengthened in the DADLE treated group, there were no statistically significant differences in the other investigated parameters between the experimental group and the control group.

Özet

[D-Ala2, D-Leu5] enkephalin (DADLE), a delta opioid receptor agonist, can induce mammalian hibernation and has hibernation-like effects in non-hibernating species. It has also been demonstrated that DADLE has protective efficacy on various tissues such as nervous, cardiac, and hepatic tissues. Despite substantial research conducted in the last decade about the tissue protective efficacy of DADLE, detailed underlying mechanisms have not been fully elucidated. In the present study we investigated in a rat model whether continuous perfusion of DADLE can induce potentially tissue protective alterations in physiological parameters. In the study 48 young male Wistar rats were used. They were divided into control and experimental groups, each group consisting of 24 animals. DADLE was perfused to experimental group rats continuously for 28 days using subcutaneously implanted osmotic mini-pumps. We investigated its effects on body temperature, antioxidant protection [total antioxidant capacity (TAC), ascorbic acid (AA), and malondialdehyde (MDA) levels], white blood cell (WBC) count, and coagulation parameters [activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT)]. While thrombin time was significantly lengthened in the DADLE treated group, there were no statistically significant differences in the other investigated parameters between the experimental group and the control group.

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Cite This Article

AKYAZI, İ., ÇÖTELİOĞLU, Ü. (2011). Chronic continuous perfusion of the hibernation triggering opioid DADLE did not cause any major physiological alterations in rats. Turkish Journal of Veterinary and Animal Sciences, Cilt: 35 - Sayı: 5, 325–330. https://ajindex.com/articles/121819

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References (7)

  1. Oeltgen, P.R., Blouin, R.A., Spurrier, W.A., Myers, R.D.: Hibernation “trigger” alters renal function in the primate. Physiol. Behav., 1985; 34: 79-81. 2. Oeltgen, P.R, Nilekani, S.P., Nuchols, P.A., Spurrier, W.A., Su, T.P.: Further studies on opioids and hibernation: delta opioid receptor ligand selectively induced hibernation in summer- active ground squirrels. Life Sci., 1988; 43: 1565-1574.
  2. Yasuma, Y., McCarron, R.M., Spatz, M., Hallenbeck, J.M.: Eff ects of plasma from hibernating ground squirrels on monocyte-endothelial cell adhesive interactions. Am. J. Physiol., 1997; 273: R1861-1869. Barnes, B.M.: Freeze avoidance in a mammal: body temperatures below 0 degree C in an Arctic hibernator. Science, 1989; 244: 1593-1595.
  3. Lyman, C.P., Chatfi eld, P.O.: Physiology of hibernation in mammals. Physiol. Rev., 1955; 35: 403-425.
  4. Chien, S., Oeltgen, P.R., Diana, J.N., Shi, X., Nilekani, S.P., Salley, R.: Two-day preservation of major organs with autoperfusion multiorgan preparation and hibernation induction trigger. J. Th orac. Cardiovasc. Surg., 1991; 102: 224. Chien, S., Oeltgen, P. R., Diana, J. N., Salley, R.K.: Extension of tissue survival time in multiorgan block preparation with a delta opioid DADLE ([D-Ala2, D-Leu5] enkephalin). J. Th orac. Cardiovasc. Surg., 1994; 107: 964. Yamanouchi, K., Yanaga, K., Okudaira, S., Eguchi, S., Furui, J., Kanematsu, T.: [D-Ala2, D-Leu5] enkephalin (DADLE) Protects Liver against Ischemia-Reperfusion Injury in the Rat. J. Surg. Res., 2003; 114: 72-77.
  5. Tsao, L.I., Ladenheim, B., Andrews, A.M., Chiueh, C.C., Cadet, J.L., Su, T.P.: Delta opioid peptide [D-Ala2,D-leu5]enkephalin blocks the long-term loss of dopamine transporters induced by multiple administrations of methamphetamine: involvement of opioid receptors and reactive oxygen species. J. Pharmacol. Exp. Th er., 1998; 287: 322-331.
  6. Borlongan C.V., Hayashi T., Oeltgen P.R., Su T.P., Wang Y.: Hibernation-like state induced by an opioid peptide protects against experimental stroke. BMC Biol., 2009; 7: 31. Gao C.J., Li, J.P., Wang, W., Lu, B.C., Niu, L., Zhu, C., Wei, Y.Y., Zhang, T., Wu, S.X., Chai, W., Li, Y.Q.: Eff ects of intracerebroventricular application of the delta opioid receptor agonist [D-Ala2, D-Leu5] enkephalin on neurological recovery following asphyxial cardiac arrest in rats. Neuroscience, 2010; 168: 531-542.
  7. Yang Y., Xia, X., Zhang, Y., Wang, Q., Li, L., Luo, G., Xia, Y.: Delta-Opioid receptor activation attenuates oxidative injury in the ischemic rat