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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 22 - Sayı: 5

Türkiye'nin Trakya Bölgesindeki Barınak Köpeklerinde Kalp Kurdu Hastalığının Serolojik ve Moleküler Prevalansı

The Serologic and Molecular Prevalence of Heartworm Disease in Shelter Dogs in the Thrace Region of Turkey [1]

HANDAN ÇETİNKAYA
İBRAHİM AKYAZI
Mete ÖZKURT
ERDAL MATUR
Pages
751–755
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This region is the transition point to Europe and obtained data in this study could contribute to the control of heartworm disease in the area.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Dirofilaria immitis is an important nematode of dogs and cats which causes fatal heartworm disease in their hosts.
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Key Findings & Data:Blood samples were collected from 402 dogs in shelters which were located in four cities (Istanbul, Edirne, Tekirdag and Kirklareli) in this region.
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Conclusion & Impact:This region is the transition point to Europe and obtained data in this study could contribute to the control of heartworm disease in the area.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Dirofilaria immitis is an important nematode of dogs and cats which causes fatal heartworm disease in their hosts. The aim of this study was to investigate the prevalence of D. immitis by serologic and molecular methods in shelter dogs, in the Thrace Region of Turkey. Blood samples were collected from 402 dogs in shelters which were located in four cities (Istanbul, Edirne, Tekirdag and Kirklareli) in this region. The blood smears were examined for the presence of D. immitis microfilariae under the light microscope. The commercial Snap3Dx test kit and PCR assay for amplifying the ITS-2 gene region were used for the serological and molecular analyses, respectively. The serologic and molecular prevalence of D. immitis was determined as 6.7% and 2.7%, respectively. D. immitis microfilariae were also determined in the blood smears of three dogs (0.8%). The distribution of the infection according to the provinces was 14.7% in Edirne, 11% in Kirklareli, 1% in Tekirdag and 0% in Istanbul. The occult infection rate was determined as 59.3%. This study revealed the presence of D. immitis for the first time in the Thrace Region of Turkey. This region is the transition point to Europe and obtained data in this study could contribute to the control of heartworm disease in the area.

Özet

Dirofilaria immitis, konaklarında ölümcül Kalp Kurdu Hastalığı'na neden olan köpek ve kedilerin önemli bir nematodudur. Bu çalışmanın amacı, Türkiye'nin Trakya Bölgesi'ndeki barınak köpeklerinde D. immitis'in prevalansının serolojik ve moleküler yöntemlerle araştırılmasıdır. Kan örnekleri, bu bölgedeki dört şehirde (İstanbul, Edirne, Tekirdağ ve Kırklareli) lokalize olmuş barınaklardaki 402 köpekten alındı. Kan yaymaları D. immitis' in mikrofilerlerinin varlığı için ışık mikroskobu altında incelendi. Snap 3Dx test kiti ve ITS-2 gen bölgesini çoğaltmak için PCR yöntemi sırasıyla serolojik ve moleküler analizlerde kullanıldı. D. immitis'in serolojik ve moleküler prevalansı sırasıyla %6.7 ve %2.7 olarak belirlendi. Ayrıca 3 köpeğin kan yaymalarında D. immitis' in mikrofilerleri saptandı (%0.8). İllere göre enfeksiyonun dağılımı Edirne'de %14.7, Kırklareli'nde %11, Tekirdağ'da %1 ve İstanbul'da %0 olarak bulundu. Gizli enfeksiyon oranı %59.3 olarak saptandı. Bu çalışma D. immitis'in varlığını, Trakya Bölgesinde ilk kez ortaya koydu. Bu bölge Avrupa'ya geçiş noktası olup, bu çalışmada elde edilen veriler Kalp Kurdu Hastalığı'nın bölgedeki kontrolüne katkı sağlayabilir.

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Cite This Article

ÇETİNKAYA, H., AKYAZI, İ., ÖZKURT, M., MATUR, E. (2016). Türkiye'nin Trakya Bölgesindeki Barınak Köpeklerinde Kalp Kurdu Hastalığının Serolojik ve Moleküler Prevalansı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 5, 751–755. https://ajindex.com/articles/272431

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