Cryopreservation of seabream (Sparus aurata) semen and fertility

Abstract

In this study, the aim was to reveal spermatological parameters of seabream (Sparus aurata), freezing ability of semen in different extenders, and fertilization capacity of frozen seabream semen. The study was conducted with 10 male and 6 female brooder seabreams. Female fish were synchronized by photoperiod and water temperature in the breeding season. Four different extenders [Mounib, Salty Mounib, Ringer, and sea water (1% NaCl)] including 10% dimethyl sulfoxide were used to assess the freezing ability of seabream semen. After postthaw spermatological examinations, three successful extenders (Mounib, Ringer, 1% NaCl) were chosen for the fertilization stage and 2 control and 3 experimental groups were formed (control I: fresh semen, control II: naturally fertilized eggs). When postthaw motility values were considered, the best results belonged to Mounib and Ringer extenders at 54.45 ± 1.18% and 58.25 ± 1.97%, respectively. At the fertilization stage, the highest hatching rates were from Mounib and Ringer at 29.20 ± 1.05% and 28.63 ± 1.12%, respectively. The statistical difference between extenders was significant (P < 0.05). Mounib and Ringer extenders gave the highest motility and fertility rates. As a result, Mounib and Ringer extenders can be used for freezing seabream semen, and semen frozen with these extenders supplies sufficient fertility results.