SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2015 / Cilt: 39 - Sayı: 1

Effect of freezing rate on goat sperm morphology and DNA integrity

Burcu ÜSTÜNER
Zekariya NUR
Selim ALÇAY
Mehmet Berk TOKER
Hakan SAĞIRKAYA
Mustafa Kemal SOYLU
Pages
110–114
Publication Date
2015-03-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen.
📊
Key Findings & Data:Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C (G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min) and stored in liquid nitrogen until use.
💡
Conclusion & Impact:Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:DNA integrity · freezing rate · goat semen

Abstract

This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C (G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 °C/min to 24 °C/min between +5 °C and -150 °C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.

Özet

This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C (G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 °C/min to 24 °C/min between +5 °C and -150 °C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.

🔖

Cite This Article

ÜSTÜNER, B., NUR, Z., ALÇAY, S., TOKER, M. B., SAĞIRKAYA, H., SOYLU, M. K. (2015). Effect of freezing rate on goat sperm morphology and DNA integrity. Turkish Journal of Veterinary and Animal Sciences, Cilt: 39 - Sayı: 1, 110–114. https://ajindex.com/articles/121706

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Cooter PZ, Goolsby HA, Prien SD. Preliminary evaluation of a unique freezing technology for bovine spermatozoa cryopreservation. Reprod Dom Animal 2005; 40: 98–99. Lemma A. Effect of cryopreservation on sperm quality and fertility. In: Manafi M, editor. Artificial Insemination in Farm Animals. Rijeka, Croatia: InTech; 2011. pp. 191–216.
  2. Kumar S, Millar JD, Watson PF. The effect of cooling rate on the survival of cryopreserved bull, ram and boar spermatozoa: a comparison of two controlled rate cooling machines. Cryobiology 2003; 46: 246–253.
  3. Kasimanickam R, Pelzer KD, Kasimanickam V, Swecker WS, Thatcher CD. Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. Theriogenology 2006; 65: 1407–1421.
  4. Amman RP, Hammerstedt RH. In vitro evaluation of semen quality: an opinion. J Androl 1993; 14: 397–406.
  5. Larson KL, DeJonge CJ, Barnes AM, Jost, LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod 2000; 15: 1717–1722.
  6. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15: 1338–1344.
  7. Hammadeh ME, Askari A, Georg T, Rosenbaum P, Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl 1999; 22: 155–162.
  8. Paasch U, Sharma RK, Gupta AK, Grunewald S, Mascha EJ, Thomas AJ Jr, Glander HJ, Agarwal A. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod 2004; 71: 1828–1837.
  9. Nur Z, Zik B, Ustuner B, Tutuncu S, Sagirkaya H, Ozguden CG, Gunay U, Dogan I. Effect of freezing rate on acrosome and chromatin integrity in ram semen. Ankara Üniv Vet Fak Derg 2011; 58: 267–272.
  10. Fraser L, Strzezek J. Effects of freezing–thawing on DNA integrity of boar spermatozoa assessed by the neutral comet assay. Reprod Dom Anim 2005; 40: 530–536.
  11. Nur Z, Zik, B, Ustuner B, Sagirkaya H, Ozguden CG. Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology 2010; 73: 1267–1275.
  12. Batista M, Ninõ T, Alamo D, Castro N, Santana M, Gonzalez F, Cabrera F, Gracia A. Successful artificial insemination using semen frozen and stored