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Journals / Turkish Journal of Veterinary and Animal Sciences / 2015 / Cilt: 39 - Sayı: 2

In vivo assessment of DNA damage in Cyprinus carpio after exposure to potassium dichromate using RAPD

Pavan KUMAR
Ravindra KUMAR
Naresh Sahebrao NAGPURE
Prakash NAUTIYAL
Basdeo KUSHWAHA
Christopher Didigwu NWANI
Wazir Singh LAKRA
Pages
121–127
Publication Date
2015-08-13
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate.
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Key Findings & Data:Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h.
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Conclusion & Impact:The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cyprinus carpio · DNA damage · potassium dichromate

Abstract

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed differences in RAPD profiles with respect to the disappearance of several bands and the appearance of new ones. There was a significant (P < 0.05) difference between 72 and 96 h after exposure with respect to genomic template stability. The variation in RAPD profiles indicated both time- and concentration-dependent relationships. The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.

Özet

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed differences in RAPD profiles with respect to the disappearance of several bands and the appearance of new ones. There was a significant (P < 0.05) difference between 72 and 96 h after exposure with respect to genomic template stability. The variation in RAPD profiles indicated both time- and concentration-dependent relationships. The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.

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Cite This Article

KUMAR, P., KUMAR, R., NAGPURE, N. S., NAUTIYAL, P., KUSHWAHA, B., NWANI, C. D., LAKRA, W. S. (2015). In vivo assessment of DNA damage in Cyprinus carpio after exposure to potassium dichromate using RAPD. Turkish Journal of Veterinary and Animal Sciences, Cilt: 39 - Sayı: 2, 121–127. https://ajindex.com/articles/121427

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