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Journals / Journal of Basic and Clinical Health Sciences / 2022 / Volume: 6 Issue: 3

Tescalcin over-expression increases Keratin 18 and 19 in K562 cells

Hande Efe(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0002-3128-0047
Özge Uysal Yoca(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0002-3906-6918
Umut Özerk Önkol(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0002-3619-1043
Zeynep Yuce(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0002-2762-0942
Pages
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Publication Date
2022-09-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.

📌 3 Key Takeaways

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Objective & Hypothesis:Tescalcin (TESC) has been shown to be essential in mammalian cells as a regulator of intracellular Ca2+.
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Key Findings & Data:Ca2+ is a second messenger functioning in many metabolic pathways as well as in cell differentiation, cell size and the cell cycle.
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Conclusion & Impact:We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Keratins · TESC · K562

Abstract

Tescalcin (TESC) has been shown to be essential in mammalian cells as a regulator of intracellular Ca2+. Ca2+ is a second messenger functioning in many metabolic pathways as well as in cell differentiation, cell size and the cell cycle. K562 cells over-expressing TESC change their morphology and adopt adherent properties. Considering differences in morphology may have been reflected in changes of the cytoskeleton, we focused on the expression levels of keratins, which are cytoskeletal intermediate filaments in epithelial cells and also expressed in K562. We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.

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Cite This Article

Efe, H., Yoca, Ö. U., Önkol, U. Ö., Yuce, Z. (2022). Tescalcin over-expression increases Keratin 18 and 19 in K562 cells. Journal of Basic and Clinical Health Sciences, Volume: 6 Issue: 3. https://doi.org/10.30621/jbachs.1101524

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