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Journals / Journal of Basic and Clinical Health Sciences / 2025 / Volume: 9 Issue: 3

Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Seher Elif Koçoğlu(ERCIYES UNIVERSITY)0000-0002-6896-2922
Leyla Çimen(Gaziantep İslam Bilim ve Teknoloji Üniversitesi)0000-0002-4730-5595
Nur Tufanoğlu(ERCIYES UNIVERSITY)0000-0002-3729-2874
Semih Yılmaz(ERCIYES UNIVERSITY)0000-0003-4835-1494
Aysun Çetin(ERCIYES UNIVERSITY)0000-0003-4959-7955
Pages
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Publication Date
2025-09-30
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusions: CAPE didn’t show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver.
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Key Findings & Data:Methods: 3 groups were formed as control (ip SF), CAPE 10 (ip 10 μmol/kg CAPE) and CAPE 50 (ip 10 μmol/kg CAPE for 5 days).
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Conclusion & Impact:Conclusions: CAPE didn’t show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:propolis · caffeic acid phenethyl ester · liver

Abstract

Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Methods: 3 groups were formed as control (ip SF), CAPE 10 (ip 10 μmol/kg CAPE) and CAPE 50 (ip 10 μmol/kg CAPE for 5 days). Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight (MW) of 53 kDa in CAPE 10 was found to be higher than control group (p<0.05). The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control (p<0.001). The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control (p<0.05). MDA levels were statistically higher in CAPE groups compared to control group (p<0.001). GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusions: CAPE didn’t show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

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Cite This Article

Koçoğlu, S. E., Çimen, L., Tufanoğlu, N., Yılmaz, S., Çetin, A. (2025). Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue. Journal of Basic and Clinical Health Sciences, Volume: 9 Issue: 3. https://doi.org/10.30621/jbachs.1588609

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