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Journals / Journal of Basic and Clinical Health Sciences / 2026 / Volume: 10 Issue: 3

Comparative Cytotoxic and Genotoxic Effects of Melatonin, Agomelatine and Ramelteon in U87-MG Glioblastoma and N2a Neuroblastoma Cells

Leyla Beytur(İNÖNÜ ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, ANATOMİ ANABİLİM DALI)0009-0007-6949-5477
Çiğdem Tekin(INONU UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL, DEPARTMENT OF HEALTH CARE SERVICES)0000-0001-9038-1924
Furkan Yüksel(NECMETTİN ERBAKAN ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, TEMEL BİLİMLER (VETERİNER) BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0002-1503-265X
Samet Öz(OSMANİYE KORKUT ATA ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0002-4549-4388
Suat Tekin(İNÖNÜ ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-2757-1802
Pages
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Publication Date
2026-09-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: These findings demonstrate that melatonin, agomelatin, and ramelteon exhibit different cytotoxic and genotoxic effects depending on the tumor cell type, highlighting their potential importance as candidate agents in neuro-oncological research.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Purpose: While melatonin has been extensively investigated for its potential antitumor activity, the effects of its receptor agonists, agomelatin and ramelteon, on neural and glial tumour cells have not been sufficiently elucidated.
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Key Findings & Data:This study aims to comparatively evaluate the cytotoxic and genotoxic effects of melatonin, agomelatin, and ramelteon on U87-MG glioblastoma and N2a neuroblastoma cells.
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Conclusion & Impact:Conclusion: These findings demonstrate that melatonin, agomelatin, and ramelteon exhibit different cytotoxic and genotoxic effects depending on the tumor cell type, highlighting their potential importance as candidate agents in neuro-oncological research.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:agomelatine · cancer · glioblastoma

Abstract

Purpose: While melatonin has been extensively investigated for its potential antitumor activity, the effects of its receptor agonists, agomelatin and ramelteon, on neural and glial tumour cells have not been sufficiently elucidated. This study aims to comparatively evaluate the cytotoxic and genotoxic effects of melatonin, agomelatin, and ramelteon on U87-MG glioblastoma and N2a neuroblastoma cells. Material and Methods: Cells were exposed to melatonin, agomelatin, and ramelteon at concentrations of 1, 5, 25, 50, and 100 µM for 24 hours. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, and genotoxicity was assessed using the comet test at half-maximum inhibitory concentrations. Results: According to the MTT results, all three compounds significantly reduced cell viability in U87-MG cells at all concentrations except 1 µM. In N2a cells, however, agomelatine and melatonin significantly reduced cell viability at all concentrations, whilst ramelteon caused a significant reduction only at concentrations of 50 and 100 µM. In the comet assay results, significant changes in parameters associated with DNA damage were observed in U87-MG cells with all three compounds. In N2a cells, whilst melatonin caused significant changes in parameters associated with DNA damage, agomelatin led to a significant reduction in cell density, and ramelteon did not induce any significant changes in the parameters assessed. The observed response patterns varied depending on the compound and cell type. Conclusion: These findings demonstrate that melatonin, agomelatin, and ramelteon exhibit different cytotoxic and genotoxic effects depending on the tumor cell type, highlighting their potential importance as candidate agents in neuro-oncological research.

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Cite This Article

Beytur, L., Tekin, Ç., Yüksel, F., Öz, S., Tekin, S. (2026). Comparative Cytotoxic and Genotoxic Effects of Melatonin, Agomelatine and Ramelteon in U87-MG Glioblastoma and N2a Neuroblastoma Cells. Journal of Basic and Clinical Health Sciences, Volume: 10 Issue: 3. https://doi.org/10.30621/jbachs.1972910

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