SavedAdd your journal

Journals / Journal of Basic and Clinical Health Sciences / 2026 / Volume: 10 Issue: 3

The Effect of Choline on Viability in Prostate Carcinoma Cells: Possible Involvement of Cholinergic Regulation

Yasemen Adalı Ruşen(İzmir University of Economics)0000-0002-8004-7364
Elif Barış(IZMIR UNIVERSITY OF ECONOMICS)0000-0001-6838-7932
Pages
—
Publication Date
2026-09-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Our preliminary results showed that choline suppressed the viability of prostate carcinoma cells at concentrations of 50µM and higher however, viability in normal prostate cells remained unchanged.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background and Purpose: Prostate carcinoma is a frequently encountered tumor, and due to its relatively long survival period, early diagnosis, treatment, and follow-up are crucial in this cancer.
📊
Key Findings & Data:Methods: DU145 human prostate epithelial carcinoma cells and PNT1a human prostate epithelial normal cell lines were treated with increasing concentrations of choline (0.5-2.5-5-25-50-75-100 µM) and viability analysis using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.
💡
Conclusion & Impact:Results: Our preliminary results showed that choline suppressed the viability of prostate carcinoma cells at concentrations of 50µM and higher however, viability in normal prostate cells remained unchanged.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Choline · Prostate · Prostate Carcinoma

Abstract

Background and Purpose: Prostate carcinoma is a frequently encountered tumor, and due to its relatively long survival period, early diagnosis, treatment, and follow-up are crucial in this cancer. Changes in the tumor microenvironment, including neural alterations, have drawn attention to their effects on treatment and prognosis. The mechanism of neural effects, such as acetylcholine (ACh), in prostate cancer is not yet fully elucidated. The aim of this research is to investigate the possible interaction of choline on cell viability in prostate carcinoma cells. The aim of this study to investigate the effects of choline in normal prostate cells and cells of prostate carcinoma. Methods: DU145 human prostate epithelial carcinoma cells and PNT1a human prostate epithelial normal cell lines were treated with increasing concentrations of choline (0.5-2.5-5-25-50-75-100 µM) and viability analysis using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Results: Our preliminary results showed that choline suppressed the viability of prostate carcinoma cells at concentrations of 50µM and higher however, viability in normal prostate cells remained unchanged. Conclusion: Although further analyses are needed, our results demonstrate that choline might have potential therapeutic effects in prostate carcinoma without effecting normal prostate cells.

🔖

Cite This Article

Ruşen, Y. A., Barış, E. (2026). The Effect of Choline on Viability in Prostate Carcinoma Cells: Possible Involvement of Cholinergic Regulation. Journal of Basic and Clinical Health Sciences, Volume: 10 Issue: 3. https://doi.org/10.30621/jbachs.1474906

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (19)

  1. Li TH, Zhao H, Peng Y, Beliakoff J, Brooks JD, Sun Z. A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells. Nucleic Acids Res 2007;35:2767–76.
  2. Kwok WK, Ling MT, Yuen HF, Wong YC, Wang X. Role of p14ARF in TWIST-mediated senescence in prostate epithelial cells. Carcinogenesis 2007;28:2467–75.
  3. Carstens RP, Eaton J V, Krigman HR, Walther PJ, Garcia-Blanco MA. Alternative splicing of fibroblast growth factor receptor 2 (FGF-R2) in human prostate cancer. Oncogene 1997;15:3059–65.
  4. Magnon C, Hall SJ, Lin J, Xue X, Gerber L, Freedland SJ, et al. Autonomic Nerve Development Contributes to Prostate Cancer Progression. Science 2013;341:1236361.
  5. Sejda A, Sigorski D, Gulczyński J, Wesołowski W, Kitlińska J, Iżycka-Świeszewska E. Complexity of Neural Component of Tumor Microenvironment in Prostate Cancer. Pathobiology 2020;87:87–99.
  6. Sigorski D, Wesołowski W, Gruszecka A, Gulczyński J, Zieliński P, Misiukiewicz S, et al. Neuropeptide Y and its receptors in prostate cancer: associations with cancer invasiveness and perineural spread. J Cancer Res Clin Oncol 2023;149:5803–22.
  7. Yilmaz Z, Eralp Inan O, Kocaturk M, Baykal AT, Hacariz O, Hatipoglu I, et al. Changes in serum proteins after endotoxin administration in healthy and choline-treated calves. BMC Vet Res 2016;12:210.
  8. Glunde K, Jacobs MA, Bhujwalla ZM. Choline metabolism in cancer: implications for diagnosis and therapy. Expert Rev Mol Diagn 2006;6:821–9.
  9. Schulthess G, Kolyvanos Naumann U, Käser L, Vetter W. (Early summer meningoencephalitis (ESME). Main symptoms: fever, headache, meningitic and encephalitic symptoms). Praxis (Bern 1994) 2006;95:91–7; quiz 98.
  10. Liu F, Xu H, Chen J, Yang B, Zhao L, Ji J, et al. Autonomic nerve regulation for prostate cancer: study based on the tissue transcriptional analysis. Transl Cancer Res 2020;9(11):6755-6767.
  11. Wang H, Zheng Q, Lu Z, Wang L, Ding L, Xia L, et al. Role of the nervous system in cancers: a review. Cell Death Discov 2021;7:76.
  12. Tibensky M, Mravec B. Role of the parasympathetic nervous system in cancer initiation and progression. Clin Transl Oncol 2021;23:669–81.
  13. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin 2021;71:209–49.
  14. Tracey KJ. Reflex control of immunity. Nat Rev Immunol 2009;9:418–28.
  15. Richman EL, Kenfield SA, Stampfer MJ, Giovannucci EL, Zeisel SH, Willett WC, et al. Choline intake and risk of lethal prostate cancer: incidence and survival. Am J Clin Nutr 2012;96:855–63.
  16. Martínez AK, Jensen K, Hall C, O’Brien A, Ehrlich L, White T, et al. Nicotine Promotes Cholangiocarcinoma Growth in Xenograft Mice. Am J Pathol 2017;187:1093–105.
  17. Terzić J, Grivennikov S, Karin E, Karin M. Inflammation and colon cancer. Gastroenterology 2010;138:2101-2114.e5.
  18. Pavlov VA, Wang H, Czura CJ, Friedman SG, Tracey KJ. The cholinergic anti-inflammatory pathway: a missing link in neuroimmunomodulation. Mol Med 2003;9:125–34.
  19. Wang H, Yu M, Ochani M, Amella C, Tanovic M, Susarla S (Seenu, et al. Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 2003;421:384–8.