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Journals / Dicle Üniversitesi Veteriner Fakültesi Dergisi / 2026 / Cilt: 19 Sayı: 1

Proliferative and Inflammatory Changes in Dogs with Pyometra Caused by Escherichia coli

Escherichia coli Kaynaklı Pyometralı Köpeklerde Proliferatif ve İnflamatuvar Değişiklikler

Aslihan Baykal Uğur(İSTANBUL ÜNİVERSİTESİ - CERRAHPAŞA, VETERİNER FAKÜLTESİ)0000-0002-2107-1874
Zeynep Günay Uçmak(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, VETERİNERLİK DOĞUM VE JİNEKOLOJİSİ ANABİLİM DALI)0000-0003-2530-1291
Merve Yılmaz(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, VETERİNERLİK DOĞUM VE JİNEKOLOJİSİ ANABİLİM DALI)0000-0002-6985-9150
Mert Sarılar(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, VETERİNERLİK DOĞUM VE JİNEKOLOJİSİ ANABİLİM DALI)0000-0003-0310-0966
İbrahim Kurban(ISTANBUL UNIVERSITY-CERRAHPASA, VETERİNER FAKÜLTESİ VOCATIONAL SCHOOL)0000-0002-8391-905X
Sevde Islamoglu(ISTANBUL UNIVERSITY-CERRAHPASA, INSTITUTE OF GRADUATE PROGRAMS)0000-0002-8838-2562
Bahar Gezer Güneş(ISTANBUL UNIVERSITY-CERRAHPASA, INSTITUTE OF GRADUATE PROGRAMS)0000-0001-8410-893X
Sinem Yaren Güzelbakan(İstanbul Üniversitesi-Cerrahpaşa Veteriner Fakültesi Patoloji Anabilim Dalı)0009-0005-3777-2738
Barış Halaç(İstanbul Üniversitesi-Cerrahpaşa Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı)0000-0002-3067-9937
Pages
29–37
Publication Date
2026-06-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the present study showed that Ki-67 is measurable in serum samples from dogs with pyometra and healthy controls using the Enzyme-Linked Immunosorbent Assay method, and that uterine tissue Ki-67 levels can serve as an effective marker reflecting proliferative changes in canine pyometra cases.

📌 3 Key Takeaways

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Objective & Hypothesis:The purpose of the current study is to evaluate the proliferative changes by measuring Ki-67 levels in both the uterus and blood serum of bitches with pyometra caused by Esherichia coli (Group PYO; n=14) and healthy diestrous controls (Group C; n=18).
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Key Findings & Data:Uterine tissue Ki-67 level in Group PYO was significantly higher than in Group C (P<0.01).
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Conclusion & Impact:In conclusion, the present study showed that Ki-67 is measurable in serum samples from dogs with pyometra and healthy controls using the Enzyme-Linked Immunosorbent Assay method, and that uterine tissue Ki-67 levels can serve as an effective marker reflecting proliferative changes in canine pyometra cases.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Ki-67 · Köpek · Pyometra

Abstract

The purpose of the current study is to evaluate the proliferative changes by measuring Ki-67 levels in both the uterus and blood serum of bitches with pyometra caused by Esherichia coli (Group PYO; n=14) and healthy diestrous controls (Group C; n=18). The study also aims to examine the relationship between proliferative changes and hematological parameters (C-reactive protein, complete blood count, albumin, serum progesterone, monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio). Uterine tissue Ki-67 level in Group PYO was significantly higher than in Group C (P<0.01). In Group PYO, albumin, red blood cell, hemoglobin, hematocrit, platelet, and platelet-lymphocyte ratio values were significantly lower compared to those in Group C, while an increase in C-reactive protein, white blood cell, neutrophil, monocyte-lymphocyte ratio, and neutrophil-lymphocyte ratio values was observed in Group PYO than in Group C. In conclusion, the present study showed that Ki-67 is measurable in serum samples from dogs with pyometra and healthy controls using the Enzyme-Linked Immunosorbent Assay method, and that uterine tissue Ki-67 levels can serve as an effective marker reflecting proliferative changes in canine pyometra cases. Also, decreased albumin, increased C-reactive protein and white blood cell counts, slight anemia, and changes in hematological ratios are considered predictive markers for evaluating inflammatory changes in pyometra cases in dogs.

Özet

Bu çalışmada, Escherichia coli kaynaklı pyometra (Grup PYO; n=14) olan dişi köpeklerin ve sağlıklı diöstrus kontrol grubunun (Grup C; n=18) uterus dokusu ve kan serumundaki Ki-67 düzeylerini ölçerek proliferatif değişikliklerin değerlendirilmesi amaçlandı. Çalışma ayrıca proliferatif değişiklikler ile hematolojik parametreler (C-reaktif protein, tam kan sayımı, albumin ve serum progesteron ile monosit-lenfosit oranı, nötrofil-lenfosit oranı, trombosit-lenfosit oranı) arasındaki ilişkiyi incelemek amacıyla yapıldı. PYO grubunun uterus dokusunda belirlenen Ki-67 seviyesi, kontrol grubuna kıyasla daha yüksek bulundu (P<0,01). PYO grubunda, albümin, kırmızı kan hücresi, hemoglobin, hematokrit, trombosit ve trombosit-lenfosit oranı değerleri ise kontrol grubuna kıyasla anlamlı olarak daha düşük olarak belirlendi, ancak C-reaktif protein, beyaz kan hücresi, nötrofil, monosit-lenfosit oranı ve nötrofil-lenfosit oranı değerlerinde PYO grubunda C grubuna kıyasla artış gözlendi. Sonuç olarak, sunulan çalışma, Ki-67'nin pyometra olan köpeklerin ve sağlıklı kontrol grubunun serum örneklerinde Enzim Bağlantılı Immünosorbent Analiz yöntemi ile ölçülebilir olduğunu ve uterus dokusu Ki-67 düzeylerinin köpek pyometra vakalarında proliferatif değişiklikleri yansıtan etkili bir belirteç olarak kullanılabileceğini göstermiştir. Ayrıca, albümin azalmasının yanında C-reaktif protein ve beyaz kan hücrelerinin artışı, hafif anemi ve hematolojik oranlardaki değişiklikler, köpeklerde pyometra vakalarında inflamatuar değişikliklerin değerlendirilmesinde öngörücü belirteçler olarak kabul edilmektedir.

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Cite This Article

Uğur, A. B., Uçmak, Z. G., Yılmaz, M., Sarılar, M., Kurban, İ., Islamoglu, S., Güneş, B. G., Güzelbakan, S. Y., Halaç, B. (2026). Proliferative and Inflammatory Changes in Dogs with Pyometra Caused by Escherichia coli. Dicle Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 Sayı: 1, 29–37. https://doi.org/10.47027/duvetfd.1872578

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