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Journals / Dicle Tıp Dergisi / 2018 / Cilt: 45 Sayı: 4

Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Mehmet Cihan Yavaş0000-0002-2923-050X
Veysi Akpolat0000-0002-2435-7800
Engin Deveci0000-0002-2353-1184
Hakki Murat Bilgin0000-0002-6040-9989
İbrahim Kaplan0000-0003-2813-1064
Ugur Seker0000-0002-1693-6378
İsmail Yildiz0000-0001-5505-838X
Esref Alkis0000-0002-3321-2873
Mustafa Salih Celik0000-0001-7397-8120
Mehmet Zülküf Akdağ0000-0003-2826-2734
Pages
447–461
Publication Date
2018-12-13
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

GI partly protects against these effects, while melatonin is more effective in this regard.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line on the testes tissue and the serum biochemistry of Wistar albino male rats.
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Key Findings & Data:Methods: In the study, 64 rats were divided into eight equal groups (n: 8).
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Conclusion & Impact:GI partly protects against these effects, while melatonin is more effective in this regard.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Melatonin · ganoderma · high voltage

Özet

Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line on the testes tissue and the serum biochemistry of Wistar albino male rats. At the same time, in the study also examined the protective effects of melatonin and ganoderma lucidum. Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, the following groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely low frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day. Results: The biochemical results of the study show an increase in total oxidant status (TOS) and oxidative stress index (OSI) level and a decrease in total antioxidant status (TAS) level in the 26 and 52 day high voltage groups, compared to the control group. In the high voltage groups, the nitric oxide (NO) value increased with the increase of the exposure period. Testicular mass (p>0.05), TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicular weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day comparisons. In routine histopathology data, we detected effects on both the 26 and 52 day HV groups, such as degeneration in spermatic cells and full structural deterioration and increasing hyalinisation. We also detected an step up in the ligament cell in the tubular field for the 52-day test groups. In melatonin added group, proliferating cell nuclear antigen (PCNA)-positive cells were more active. TUNEL assay analysis showed that there was no significant difference between the the 26 day experiment groups (p>0.05), but that there was a significant dissimilarity between the 52 day experiment groups (p<0.05). Conclusions: In our study, the oxidative effect of created by a high voltage results in changes in serum biochemistry of rats and a number of degenerative deterioration in the histological structure of testes. Aslo, it is shown that the oxidative effect created by a high voltage has negative effects on the creation of spermatogonia. GI partly protects against these effects, while melatonin is more effective in this regard.

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Cite This Article

Yavaş, M. C., Akpolat, V., Deveci, E., Bilgin, H. M., Kaplan, İ., Seker, U., Yildiz, İ., Alkis, E., Celik, M. S., Akdağ, M. Z. (2018). Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Tıp Dergisi, Cilt: 45 Sayı: 4, 447–461. https://doi.org/10.5798/dicletip.497923

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