Both of the monolayer and 1% of suspaded cells (In ~ Hanks'Balanced Salt Solution containing 0.5 per cent Lactalbumin Hydrolysate)were prepared with the minced and trypsinized tissues ofSheep kidney cortex.These cells were infected with Foot and Mouth disease virus(li,MDV) type SATı which is obtained from the State Fann, Çiftelerand adapted in tissue culture.The titer conducted in tissue culture with the virus cultivatedby this method was LD50 l04The result of titration in suckling mice was unsatisfactory.It can be said that one can get a large amount of virus and savetime and material with the suspended celles method opposed tothe monolayer method.
📌 3 Key Takeaways
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Objective & Hypothesis:Both of the monolayer and 1% of suspaded cells (In ~ Hanks'Balanced Salt Solution containing 0.5 per cent Lactalbumin Hydrolysate)were prepared with the minced and trypsinized tissues ofSheep kidney cortex.These cells were infected with Foot and Mouth disease virus(li,MDV) type SATı which is obtained from the State Fann, Çiftelerand adapted in tissue culture.The titer conducted in tissue culture with the virus cultivatedby this method was LD50 l04The result of titration in suckling mice was unsatisfactory.It can be said that one can get a large amount of virus and savetime and material with the suspended celles method opposed tothe monolayer method.
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Key Findings & Data:Both of the monolayer and 1% of suspaded cells (In ~ Hanks'Balanced Salt Solution containing 0.5 per cent Lactalbumin Hydrolysate)were prepared with the minced and trypsinized tissues ofSheep kidney cortex.These cells were infected with Foot and Mouth disease virus(li,MDV) type SATı which is obtained from the State Fann, Çiftelerand adapted in tissue culture.The titer conducted in tissue culture with the virus cultivatedby this method was LD50 l04The result of titration in suckling mice was unsatisfactory.It can be said that one can get a large amount of virus and savetime and material with the suspended celles method opposed tothe monolayer method.
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Conclusion & Impact:Both of the monolayer and 1% of suspaded cells (In ~ Hanks'Balanced Salt Solution containing 0.5 per cent Lactalbumin Hydrolysate)were prepared with the minced and trypsinized tissues ofSheep kidney cortex.These cells were infected with Foot and Mouth disease virus(li,MDV) type SATı which is obtained from the State Fann, Çiftelerand adapted in tissue culture.The titer conducted in tissue culture with the virus cultivatedby this method was LD50 l04The result of titration in suckling mice was unsatisfactory.It can be said that one can get a large amount of virus and savetime and material with the suspended celles method opposed tothe monolayer method.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Şap · Şap Virusunun Üretilmesi
Abstract
Both of the monolayer and 1% of suspaded cells (In ~ Hanks'Balanced Salt Solution containing 0.5 per cent Lactalbumin Hydrolysate)were prepared with the minced and trypsinized tissues ofSheep kidney cortex.These cells were infected with Foot and Mouth disease virus(li,MDV) type SATı which is obtained from the State Fann, Çiftelerand adapted in tissue culture.The titer conducted in tissue culture with the virus cultivatedby this method was LD50 l04The result of titration in suckling mice was unsatisfactory.It can be said that one can get a large amount of virus and savetime and material with the suspended celles method opposed tothe monolayer method.
Özet
Koyun böbreği kortex kısmı kıyılıp, tripsinize edilerek elde olunanhücre ile hem manolayer doku kültürü, hem de % 1 nisbetindeLactalbumin Hydrolysate'lı Hanks' vasatında (0,5 % lactalbuminhydrolysate ihtiva eden) suspanse hücre kültürü yapıldı. Bu kültürÇifteler harasından elde edilen doku kültürüne adapte SATı şap virusuile enfekte edildi. Bu metodla üretilen vinısun doku kültüründeyapılan titrasyonunda LD50 104 titre alındı. Beyaz yavru farelerdeyapılan titrasyonda ise iyi sonuç alınamadı. Zaman ve malzemedenkazanmak suretiyle manolayer metoda nazaran çok virus elde olacağı söylenebilir.
SÜTÇÜ, M., ŞENTÜRK, M., ÜNLÜLEBLEBİCİ, N. (1963). The cultivation of FMD virus in the sheep kiddney suspended cells. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 2 - Sayı: 2, 87–93. https://ajindex.com/articles/118746