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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2019 / Cilt: 30 - Sayı: 1

Metagenomic Analysis of the Early Ripened Continental Cheese Microbiome

Erken Olgunlaşma Dönemindeki Geleneksel Peynir Mikrobiyomunun Metagenomik Analizi

Sadık SAVAŞAN
Devrim BEYAZ
Pages
27–35
Publication Date
2019-06-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The all species showed more than 99% homology to the referance genes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, microbiota of early ripened continental cheeses from raw sheep milk collected from moun-tain villages was determined by next-generation sequencing and metagenomic analysis.
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Key Findings & Data:Firmicutes was found as the dominant phylum by the avearge of 91,5%, when Actinobacteria was found as the minor phylum (0,01%).
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Conclusion & Impact:The all species showed more than 99% homology to the referance genes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Peynir · mikrobiyom · mikrobiyota

Abstract

In this study, microbiota of early ripened continental cheeses from raw sheep milk collected from moun-tain villages was determined by next-generation sequencing and metagenomic analysis. Firmicutes was found as the dominant phylum by the avearge of 91,5%, when Actinobacteria was found as the minor phylum (0,01%). By the metagenomic analysis of cheese samples 25 genuses were determined, 23 of these genuses were found in all of the cheese samples. In the genus averages, the highest diversity was viewed in Enterobacteriaceae family as 13 genus. Lactococcuc lactis was determined as dominant (avr.84%) species in all samples. The all species showed more than 99% homology to the referance genes.

Özet

Bu araştırmada, dağ köylerinde çiğ koyun sütünden üretilen geleneksel peynirlerin erken olgunlaşma döneminde alınan örneklerinin yeni nesil sekanslama ve metagenomik analiz ile mikrobiyotaları saptanmıştır. Firmicutes ortalama %91,5 ile en yoğun filum olarak bulunurken, Actinobacteria en seyrek filum olarak (%0,01) bulundu. Peynir örnekle-rinin metagenomik analizi sonucunda 25 cins belirlenirken bunlardan 23’ü tüm örneklerde ortak olarak bulundu. Cins düzeyinde en yüksek çeşitlilik 13 cins ile Enterobacteriaceae familyası içinde görüldü. Tüm örneklerde en baskın tür Lactococcuc lactis (ort.%84) olarak belirlendi. Tüm türler referans genlere %99’un üzerinde homoloji gösterdi.

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Cite This Article

SAVAŞAN, S., BEYAZ, D. (2019). Metagenomic Analysis of the Early Ripened Continental Cheese Microbiome. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 30 - Sayı: 1, 27–35. https://ajindex.com/articles/118577

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