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Journals / Uludağ Üniversitesi Tıp Fakültesi Dergisi / 2026 / Cilt: 52

The Modulatory Role of Uridine on Nutrition-Related Molecules in a Rat Model of Polycystic Ovary Syndrome

Polikistik Over Sendromu Modeli Oluşturulan Sıçanlarda Üridinin Beslenme ile İlişkili Moleküller Üzerine Etkisi

Elanur Üstünbaş(Bursa Uludag University, Graduate School of Health Sciences, Department of Medicine- Physiology, University Street, 16059, Bursa, Türkiye.)0009-0009-9405-6753
Süeda Tunçak(Bursa Uludag University, Faculty of Medicine, Department of Physiology, University Street, 16059, Bursa, Türkiye.)0000-0002-5723-0769
Zehra Selen Mutlu(Bursa Uludag University, Graduate School of Health Sciences, Department of Medicine- Physiology, University Street, 16059, Bursa, Türkiye.)0000-0002-2370-4698
Cihan Çakır(Bursa Uludag University, Faculty of Medicine, Department of Histology and Embryology, University Street, 16059, Bursa, Türkiye.)0000-0002-8332-7353
Ayşen Çakır(Bursa Uludag University, Faculty of Medicine, Department of Physiology, University Street, 16059, Bursa, Türkiye.)0000-0001-7729-7373
Pages
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Publication Date
2026-07-08
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results demonstrated that decreased orexin-A and increased leptin levels observed in the PCOS group were significantly altered following uridine treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Polycystic Ovary Syndrome (PCOS) is a complex hormonal and metabolic disorder affecting approximately 10% of women of reproductive age and is characterized by infertility, insulin resistance, and hyperandrogenism.
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Key Findings & Data:Peptides involved in energy homeostasis, such as leptin, nesfatin-1, and orexin-A, also play critical roles in the pathophysiology of PCOS.
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Conclusion & Impact:The results demonstrated that decreased orexin-A and increased leptin levels observed in the PCOS group were significantly altered following uridine treatment.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Polikistik over sendromu · Üridin · Nesfatin-1

Abstract

Polycystic Ovary Syndrome (PCOS) is a complex hormonal and metabolic disorder affecting approximately 10% of women of reproductive age and is characterized by infertility, insulin resistance, and hyperandrogenism. Peptides involved in energy homeostasis, such as leptin, nesfatin-1, and orexin-A, also play critical roles in the pathophysiology of PCOS. It has been suggested that the nucleoside uridine may exert regulatory effects on these peptides. The aim of this study was to investigate the effects of uridine on nesfatin-1, orexin-A, and leptin in a rat model of PCOS. In this study, 35 female Sprague-Dawley rats aged 8-10 weeks and weighing 200-300g were used. To induce the PCOS model, rats received subcutaneous injections of dehydroepiandrosterone at a dose of 60mg/kg/ml for 21 consecutive days. Following this period, uridine was administered intraperitoneally at doses of 250 or 500mg/kg/ml for a duration equivalent to one estrous cycle (5days) in the relevant groups. After decapitation, blood samples were collected, centrifuged, and serum samples were obtained. Serum levels of orexin-A, nesfatin-1, and leptin were measured using ELISA. Intergroup comparisons were performed using one-way analysis of variance, followed by the Holm-Sidak post hoc test. p<0.05 was considered statistically significant. The results demonstrated that decreased orexin-A and increased leptin levels observed in the PCOS group were significantly altered following uridine treatment. In addition, nesfatin-1 levels did not show a significant difference among the groups. In conclusion, modulation of disrupted orexin-leptin balance by uridine in PCOS may provide a novel perspective for management of metabolic complications associated with the syndrome.

Özet

Polikistik Over Sendromu (PKOS), üreme çağındaki kadınların yaklaşık %10'unu etkileyen, infertilite, insülin direnci ve hiperandrojenizm ile karakterize karmaşık hormonal ve metabolik bir bozukluktur. Enerji dengesini etkileyen leptin, nesfatin-1 ve oreksin-A gibi peptitler PKOS patofizyolojisinde de kritik rol oynamaktadır. Üridin nükleozidinin bu peptitler üzerinde düzenleyici bir etkisi olabileceği de bilinmektedir. Bu çalışmanın amacı PKOS modeli oluşturulmuş sıçanlarda üridin uygulanmasının nesfatin-1, oreksin-A ve leptin gibi beslenme ile ilişkili moleküller üzerine etkisini incelemektir. Bu çalışmada; 8-10 haftalık, 200-300 gram ağırlığında 35 adet dişi Sprague-Dawley sıçan kullanılmıştır. PKOS modelini indüklemek için sıçanlara 21 gün boyunca her gün 60 mg/kg/ml dozunda dehidroepiandrosteron (DHEA) subkutan yolla uygulanmıştır. 21 günlük sürenin ardından, farklı üridin dozları (250 veya 500 mg/kg/ml) bir östrus döngüsüne (5 gün) eşdeğer sürede ilgili gruplara intraperitonal yolla uygulanmıştır. Dekapitasyon sonrası kan örnekleri santrifüj edilerek serumları toplanmıştır. Elde edilen serumlardan ELISA yöntemi ile oreksin-A, nesfatin-1 ve leptin düzeyleri tayin edilmiştir. Gruplar arası karşılaştırmalarda Tek Yönlü ANOVA (One-Way ANOVA) ve ardından post-hoc Holm-Sidak testi kullanılmış; p<0,05 değeri anlamlı kabul edilmiştir. PKOS grubunda azalan oreksin-A ve artan leptin seviyelerinin, üridin tedavisi ile anlamlı şekilde değiştiği gözlemlenmiştir. Nesfatin-1 seviyeleri ise gruplar arasında belirgin bir değişim göstermemiştir. Sonuç olarak, PKOS'ta bozulan oreksin-leptin dengesinin üridin ile modüle edilmesi, sendromun metabolik komplikasyonlarının tedavisinde yeni bir bakış açısı sunmaktadır.

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Cite This Article

Üstünbaş, E., Tunçak, S., Mutlu, Z. S., Çakır, C., Çakır, A., *, A. Ç. (2026). The Modulatory Role of Uridine on Nutrition-Related Molecules in a Rat Model of Polycystic Ovary Syndrome. Uludağ Üniversitesi Tıp Fakültesi Dergisi, Cilt: 52. https://doi.org/10.32708/uutfd.1885084

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