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Journals / Kocatepe Veteriner Dergisi / 2026 / Cilt: 12 Sayı: 1

Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation

Kısa Süreli Saklanan Koç Spermasında 7-Dehidrokolesterolün Spermatolojik Parametrelere Etkisi

Muhammed Enes İnanç(VETERİNER FAKÜLTESİ)0000-0001-6954-6309
Şükrü Güngör(VETERİNER FAKÜLTESİ)
Ayhan Ata(VETERİNER FAKÜLTESİ)
Pages
15–19
Publication Date
2019-03-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4 ° C preservation.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen spermatological parameters on short term preservation at +4 ⁰ C.
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Key Findings & Data:Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x10 6 ) and control (C).
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Conclusion & Impact:In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4 ° C preservation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:7-dehidrokolesterol · siklodekstrin · motilite

Abstract

The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen spermatological parameters on short term preservation at +4 ⁰ C. Collected semen with electroejaculator at non-breeding season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x10 6 ) and control (C). After extended semen samples were preservation at +4 ⁰ C and evaluated motility, membrane integrity and abnormal spermatozoa rate until 96. hour. There were no significant differences between the groups on abnormal spermatozoa rate (P>0.05); there were a significant differences between the groups on motility at all storage period (P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96. hour (P<0.05). The highest dose (5 mg/120x10 6 ) 7-DCLC were decreased the motility and membrane integrity compared to other groups. Besides, 7-DCLC 3.0 mg group membrane integrity was lower than control at 72. hour (P<0.05). In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4 ° C preservation.

Özet

Bu çalışmanın amacı, 7-dehidrokolesterol yüklenmiş siklodekstrinin (7-DCLC) +4 C ⁰ ’ de k ı sa s ü reli saklanan ko ç spermas ı n ı n spermatolojik parametrelerine etkisinin ara ş t ı r ı lmas ı d ı r. Her bir hayvandan spermalar elektroejak ü lat ö r yard ı m ı ile sezon d ışı nda al ı narak d ö rt eşit gruba ayrıldı, farklı oranlarda 7-DCLC (1,5 mg, 3,0 mg and 5 mg/120x10 6 ) içeren ve içermeyen kontrol (C) tris/yumurta sarısı sulandırıcı ile sulandırıldı. Sulandırılan spermalar, +4 C ⁰ ’ de saklanarak 96 saat s ü resince motilite, membran bütünlüğü ve anormal spermatozoa yönünden incelendi. Saklama süresi boyunca anormal spermatozoa yönünden gruplar arasında bir farklılık bulunmazken (P>0,05); motilite açısından gruplar arasında farklılıklar tespit edildi (P<0,05). 72. ve 96. zaman dilimlerinde en yüksek motilite 7-DCLC 1,5 mg/120x10 6 grubunda tespit edildi (P<0,05). En yüksek dozlu (5 mg) 7-DCLC grubu diğer gruplarla karşılaştırıldığında spermatozoa motilitesini ve membran bütünlüğünü düşürdüğü tespit edildi. Ayrıca, 72. saatte 7-DCLC 3,0 mg grubunun membran bütünlüğü kontrol grubuna göre daha az olduğu tespit edildi (P<0,05). Sonuç olarak, 7-DCLC 1,5 mg içeren tris/yumurta sarısı sulandırıcısı koç spermasında motiliteyi ve membran bütünlüğünü +4 C ⁰ ’ de 96 saat s ü resince korudu ğ u belirlendi.

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Cite This Article

İnanç, M. E., Güngör, Ş., Ata, A. (2019). Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veteriner Dergisi, Cilt: 12 Sayı: 1, 15–19. https://doi.org/10.30607/kvj.492556

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