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Journals / Mediterranean Agricultural Sciences / 1997 / Volume: 10 Issue: 1

Genetic Analysis of Certain Allium Species with RAPD-PCR

Bazı Allium Türlerinin RAPD-PCR ile Genetik Analizleri

Leyla Açık
Bülent Samancı
Mehmet Yapar
Ayhan Kubar
Pages
136–142
Publication Date
1997-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Genetic distances between A. neopolitanum, A. callidictyon, A. rupestre, A. scorodopr asam, A. affine and A. junceum were calculated.

📌 3 Key Takeaways

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Objective & Hypothesis:Ran dom amplified polymorphic DNA (RAPD) markers were used to identify and distinguish between several Turkish Allium species: A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasımı, A. affine and A. junceum.
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Key Findings & Data:The primers A10 were evaluated for their usefulness.
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Conclusion & Impact:Genetic distances between A. neopolitanum, A. callidictyon, A. rupestre, A. scorodopr asam, A. affine and A. junceum were calculated.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:RAPD-PCR · Genetik Analiz · Dendogram

Abstract

Ran dom amplified polymorphic DNA (RAPD) markers were used to identify and distinguish between several Turkish Allium species: A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasımı, A. affine and A. junceum. The primers A10 were evaluated for their usefulness. Reactions with primer A 10 produced amplified band and the size of amplified DNA fragments ranged from 100 to 947 base pairs. Reactions with another group of 11 primers made possible the separation of some but not all species. Genetic distances between A. neopolitanum, A. callidictyon, A. rupestre, A. scorodopr asam, A. affine and A. junceum were calculated.

Özet

RAPD belirleyicileri Türkiye'de bulunan Allium türlerini ayırmak ve belirlemek için kullanıldı: A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasıun, A. affine ve A. junceum. A10 primeri faydalılığı için değerlendirildi. A10 primeri ile reaksiyonlar amplifike olmuş band üretti ve amplifike olmuş DNA parçalarının büyüklüğü 100-947 baz çifti arasında değişti. Diğer 11 primer grubu ile reaksiyonlar bazı türlerin ayrışımını kolaylaştırdı. A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasum, A. affine ve A. junceum arasındaki genetik uzaklıklar hesaplandı.

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Cite This Article

Açık, L., Samancı, B., Yapar, M., Kubar, A. (1997). Genetic Analysis of Certain Allium Species with RAPD-PCR. Mediterranean Agricultural Sciences, Volume: 10 Issue: 1, 136–142. https://ajindex.com/articles/1170811

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