SavedAdd your journal

Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1995 / Cilt: 8 Sayı: 1

Comparison of Fluorescence Antıbody Technique, Mouse lnoculation Test and Rabies Tissue Culture lnfection Testsin Laboratory Diagnosis of Rabies

Kuduz Hastalığının Laboratuvar Teşhisinde Floresan Antikor, Fare İnokulasyon Testi ve Kuduz Doku Kültürü Enfeksiyon Testlerinin Karşılaştırılması

Tülin Güzel(T.C. TARIM VE ORMAN BAKANLIĞI)
Orhan Aylan(T.C. TARIM VE ORMAN BAKANLIĞI)
Pages
45–55
Publication Date
1995-12-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Only 2 materials being positive for FAT and MIT, were negative for RTCiT. 141 specimens gave similar results each three techniques.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, comparative application of FAT, MIT and RTCIT are aimed and used for the purpose of laboratory diagnosis of rabies that the materials are submitted to Rabies Diagnosis Laboratory of Etlik Veterinary Control and Research Institute.
📊
Key Findings & Data:In this study, 143 materials belonging 14 province and their districts were tested.
💡
Conclusion & Impact:Only 2 materials being positive for FAT and MIT, were negative for RTCiT. 141 specimens gave similar results each three techniques.
🔬Design:Araştırma Makalesi
📍Scope:141 specimens
⚙️Variables:Kuduz · Kuduz Teşhisi · Kuduz Teşhisinde Floresan Antikor Testi

Abstract

In this study, comparative application of FAT, MIT and RTCIT are aimed and used for the purpose of laboratory diagnosis of rabies that the materials are submitted to Rabies Diagnosis Laboratory of Etlik Veterinary Control and Research Institute. In this study, 143 materials belonging 14 province and their districts were tested. Of these 143 brain specimens tested by different three techniques, 23 positives and 118 negatives were found. Only 2 materials being positive for FAT and MIT, were negative for RTCiT. 141 specimens gave similar results each three techniques.

Özet

Bu araştırmada T.K.I.B. Etlik Veteriner Kontrol ve Araştırma Enstitüsü Kuduz Teşhis Laboratuvarına gönderilen materyallerde, kuduzun teşhisinde kullanılan laboratuvar yöntemlerinden FAT, Fare Inakulasyon Testi ve Kuduz Doku Kültürü Enfeksiyon Testlerinin karşılaştırmalı olarak çalışılması amaçlanmıştır . Araştırmada 14 il ve ilçelerinden gönderilen 13 farklı hayvan türüne ait 143 adet materyal test edilmiştir. üç ayrı teknikle test edilen 143 adet beyin numunesinin 23'ü pozitif, 118'i de negatif sonuç vermiştir. FAT ve Fare inokulasyon Testi ile pozitif sonuç veren 2 numune Kuduz Doku Kültürü Enfeksiyon Testi ile negatif sonuç vermiştir. 141 numune her üç teknikle de benzer sonuç vermiştir.

🔖

Cite This Article

Güzel, T., Aylan, O. (1995). Comparison of Fluorescence Antıbody Technique, Mouse lnoculation Test and Rabies Tissue Culture lnfection Testsin Laboratory Diagnosis of Rabies. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 8 Sayı: 1, 45–55. https://ajindex.com/articles/1166654

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (27)

  1. BARRAT. J., BARRAT, M.J., PICARD, M., AUBERT, MFA: Diagnosis of rabies on cell culture: comparison of the results of ·inoculation of a murine neuroblastoma cell line and mouse inoculotion. Comp. Imm. Microb. and Inf. Dis. 11: 207-214,1988.
  2. BOURHY, H., ROLLIN, P.E., VINCENT. J., SUREAU, P.: Comparative field evaluation of the fluorescent antibody test, virus isolation from tissue culture and enzyme immunodiagnosis for rapid laboratory diagnosis of rabies. J. Clin. Microbiol. 27: 519-523, 1989.
  3. BURGU, 1.: Özel Viroloji, Teksir. AÜ. Vet Fak., 1989.
  4. BUXTON, A, FRASER, G.: Rhabdoviruses. Animal Microbiology. Blackwell Scientific Publication, Edinburgh pp. 553-567, 1977.
  5. COONS, AH., KAPLAN, M.H.: Localization of antigen in tissue cells. IL lmprovements in a methodefor the detection of antigen by means of fluorescent antibody. J.Exp.Med. 91: 1-13, 1950.
  6. CRICK, J., KING, A.: Culture of Rabies Virus in vitro. In: Campbell, J.B., Charlton, K.M.: Rabies Kluwer Academic Publishers. U.S.A. pp. 47-65, 1988.
  7. DEAN, D.J., ABELSETH, M.K.: The fluorescent antibody test. In: Kaplan, M.M., Koprowski, H., Laboratory Techniques in Rabies W. H. O. Monograph 23, 3rd. ed. 73-84, 1973.
  8. DELETOILLE, P.: La rage et son diagnostic. Labarama Diagnostics Pasteur, 27: 6-11, 1988.
  9. GILLESPIE, J.H., TIMONEY, J.F.: Hagan and Brunner's lnfectious Diseases of Domestic Animals 7th. ed., Cornell Universty Press, pp. 758-781, 1981.
  10. GOLDWASSER, R.A., KISSLING, R.E.: Fluorescent antibody staining of street and fıxed rabies virus antigens. Proc. Soc. Exp. Bio. and Med., 98:219- 223, 1958.
  11. GÜLEY, M., TUNUS, M.M., TOKER, M., ŞENTÜRK, M.: FloresanAntikor Tekniği ile kuduz teşhisi. Etlik Vet. Bakt. Enst. Dergisi 3(6): 104-108, 1968.
  12. KOPROVVSKI, H.: The mouse inceulation test In: Kaplan, M.M., Koprowski, H. Laboratory ·Tecniques in Rabies. W. H. O. Monograph 23, 3rd. ed. 85-93, 1973.
  13. Mc. QUEEN, J.L., LEWIS, A.L., SCHNEIDER, N.J.: Rabies diagnosis by fluorescent antibody 1. lt's evaluation a public health laboratory. American Journal Public Health 50: 1743-1752, 1960.
  14. RUDD, R.J., TRIMARCHI, C.V.: Comparison of sensitivity of BHK-21 and Murine Neuroblastoma cells in the isolation of street strain rabies virus. J. Clin. Microbiol. 25: 1456-1458, 1987.
  15. RUDD, R.J., TRIMARCHI, C.V.: Development and evaluation of an in vitro virus isolation procedure as a replacement for the mouse inceulation test in rabies diagnosis. J.Ciin.Microbiol. 27: 2522-2528, 1989.
  16. TIERKEL, E.S.: Rapid microscopic examination for Negri bodies preparation of specimens for biological test. In: Kaplan, M. M. Koprowski, H. Laboratory Techniques in Rabies. W.H.O. Monograph 23.3rd. ed. 73-93, 1973.
  17. WEBSTER, W.A.: A tissue culture infection test in routine rabies diagnosis. Can. J. Vet. Res. 51: 367-369, 1987.
  18. WORLD HEALTH ORGANIZATION:Rapid Rabies Enzyme 1 mmunodiagnosis Kit. Bu iletin of the World Health Organization88.29: 1-15, 1988.
  19. BARRAT. J., BARRAT, M.J., PICARD, M., AUBERT, MFA: Diagnosis of rabies on cell culture: comparison of the results of ·inoculation of a murine neuroblastoma cell line and mouse inoculotion. Comp. Imm. Microb. and Inf. Dis. 11: 207-214,1988.
  20. COONS, AH., KAPLAN, M.H.: Localization of antigen in tissue cells. IL lmprovements in a methodefor the detection of antigen by means of fluorescent antibody. J.Exp.Med. 91: 1-13, 1950.
  21. CRICK, J., KING, A.: Culture of Rabies Virus in vitro. In: Campbell, J.B., Charlton, K.M.: Rabies Kluwer Academic Publishers. U.S.A. pp. 47-65, 1988.
  22. GILLESPIE, J.H., TIMONEY, J.F.: Hagan and Brunner's lnfectious Diseases of Domestic Animals 7th. ed., Cornell Universty Press, pp. 758-781, 1981.
  23. KOPROVVSKI, H.: The mouse inceulation test In: Kaplan, M.M., Koprowski, H. Laboratory ·Tecniques in Rabies. W. H. O. Monograph 23, 3rd. ed. 85-93, 1973.
  24. Mc. QUEEN, J.L., LEWIS, A.L., SCHNEIDER, N.J.: Rabies diagnosis by fluorescent antibody 1. lt's evaluation a public health laboratory. American Journal Public Health 50: 1743-1752, 1960.
  25. RUDD, R.J., TRIMARCHI, C.V.: Development and evaluation of an in vitro virus isolation procedure as a replacement for the mouse inceulation test in rabies diagnosis. J.Ciin.Microbiol. 27: 2522-2528, 1989.
  26. TIERKEL, E.S.: Rapid microscopic examination for Negri bodies preparation of specimens for biological test. In: Kaplan, M. M. Koprowski, H. Laboratory Techniques in Rabies. W.H.O. Monograph 23.3rd. ed. 73-93, 1973.
  27. WEBSTER, W.A.: A tissue culture infection test in routine rabies diagnosis. Can. J. Vet. Res. 51: 367-369, 1987.