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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2026 / Cilt: 8 Sayı: 4

The Determination Of The Effect Of Freezing-Thawing On The Serum Antibody Titters For IBR Virus BY SNT and ELİSA

Dondurup Çözdürmenin Serumdaki IBR Virus Antikor Titrelerine Etkisinin SNT ve ELİSA ile Tesbiti

Özden Kabaklı(Veteriner Kontrol Merkez Araştırma Enstitü Müdürlüğü)
Ayhan Akçora(Veteriner Kontrol Merkez Araştırma Enstitü Müdürlüğü)
Pages
169–180
Publication Date
1996-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed no signifıcant change on antibody levels in sera, even after 10 cyles of freezİng and thawing.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, 250 serum samples were obtanied from cattle which infected with IBR/IPV virus, in various regions of Turkey.
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Key Findings & Data:In addition to, 44 serum samples selected in 250 senım which tested before.
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Conclusion & Impact:The results showed no signifıcant change on antibody levels in sera, even after 10 cyles of freezİng and thawing.
🔬Design:Araştırma Makalesi
📍Scope:80 samples
⚙️Variables:IBR · Virus · Antikor

Abstract

In this study, 250 serum samples were obtanied from cattle which infected with IBR/IPV virus, in various regions of Turkey. They were tested for IBRJ IPV virus antibodies by microneutralization test and ELISA. Two tests were compared for the detection ofthe proportion ofpositive sera. In addition to, 44 serum samples selected in 250 senım which tested before. They were frozen/ thawed 10 times and tested for effects of freezİng thawing on antibody titers in sera by SNT and ELISA. In ELISA 173 of 250 sera were estimated as positive and 77 were negative. For SNT test 170 were positive, 80 were negative. Three of 80 samples were negative for SNT and positive in the ELISA. Using the Chi square method the proportion ofpositive sera in both tests were X2=236 (p<O. 001) . The relative sensitivity and specifıcity of ELI SA in comparison with the SNT were 100% and 96.25%, respectively. In this study, 40 positive sera of 44 which 10 times frozenlthawed and tested before by ELI SA and MN test were found to have 1 : 1 .41 - 1:316 neutralising dose 50 and in ELISA 50%-95% OD values of antibodies. There was a good corelation (r=0.90) between ELI SA and SNT. The results showed no signifıcant change on antibody levels in sera, even after 10 cyles of freezİng and thawing.

Özet

Bu araştırmada Türkiye'nin değişik bölgelerinden IBR/IPV şüpheli sığırlardan alınan 250 adet serum hem mikro nötralizasyon testi hemde ELISA ile test edildi. Testler arasındaki farklılık karşılaştırıldı. Ayrıca test edilen bu 250 serum içinde 44 adedi 10 kez dondurulup - çözdüri.ilerek, dondurup çözdürmenin serumlardaki antikor seviyeleri üzerine etkisi SNT ve ELISA ile araştırıldı. ELI SA ile 250 adet kan serumunun 173 adedi pozitif, 77 adedi negatif sonuç verdi. SNT ile 170 adedi pozitif, 80 adedi negatif sonuç verdi. SNT ile negatif sonuç veren 80 serumdan 3 adedi ELISA ile pozitifreaksiyon verdi . Chi square (X2) metodu kullanılarak her iki testte serumlardaki pozitiflik oranı karşılaştınldığındaX2=236(p<O . OO 1) bulundu. SNT standartkabul edilerek ELISA karşılaştırıldığında spesifıtesi %96.25, sensitivitesi %100 olarak bulundu. Bu araştırmada, 10 kez dondurulup-çözdüri.ilen 44 adet serumun başlangıçta her iki test ile pozitif oldukları tesbit edilen 40 adedinin serum nötralizasyon değerleri (SN50) 1:1.41 -1:316, ELISA'daki optikal dansite (OD) değerleri %50 - %95 arasında dağılım gösterdiği bulundu. Her iki test arasında iyi bir pozitif korelasyon ( r=O . 90) bulunduğu tesbit edildi. Elde edilen sonuçlar, 1 O kez dondunıp- çözdümlenin bile serumdaki antikorlar üzerinde önemli bir değişikliğe sebep olmadığını gösterdi.

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Cite This Article

Kabaklı, Ö., Akçora, A. (1996). The Determination Of The Effect Of Freezing-Thawing On The Serum Antibody Titters For IBR Virus BY SNT and ELİSA. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 8 Sayı: 4, 169–180. https://ajindex.com/articles/1166648

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