T. equigenitalis was not isolated from any of the samples and was not detected by direct-PCR.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:In this study, classical culture method and a direct polymerase chain reaction (direct-PCR) assay was applied to urethral fossa and sinus swabs taken from 25 thoroughbred stallion racehorses to detect Taylorella equigenitalis responsible for contagious equine metritis (CEM).
📊
Key Findings & Data:In this study, classical culture method and a direct polymerase chain reaction (direct-PCR) assay was applied to urethral fossa and sinus swabs taken from 25 thoroughbred stallion racehorses to detect Taylorella equigenitalis responsible for contagious equine metritis (CEM).
💡
Conclusion & Impact:T. equigenitalis was not isolated from any of the samples and was not detected by direct-PCR.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Culture · direct-PCR · stallion
Abstract
In this study, classical culture method and a direct polymerase chain reaction (direct-PCR) assay was applied to urethral fossa and sinus swabs taken from 25 thoroughbred stallion racehorses to detect Taylorella equigenitalis responsible for contagious equine metritis (CEM). T. equigenitalis was not isolated from any of the samples and was not detected by direct-PCR.
Özet
Bu çalışmada 25 safkan yarış aygırından alınan uretral fossa ve sinus svaplarında, direkt polimeraz zincir reaksiyonu (direkt-PCR) ve klasik kültür yöntemleri ile kontagiyöz equine metritis (CEM)’in etkeni olan Taylorella equigenitalis varlığı aranmıştır. Alınan örneklerden T. equigenitalis izole edilemediği gibi direkt-PCR yöntemi ile de tespit edilememiştir.
Müştak, H. K., Günaydın, E., Dakman, A., Küçükayan, U. (2009). Investigation of Taylorella equigenitalis from thoroughbred stallion genital swabs by direct polymerase chain reaction and culture method. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 20 Sayı: 1-2, 19–22. https://ajindex.com/articles/1166485
Anzai T, Eguchi M, Sekizaki T, Kamada M, Yamamoto K, Okuda T, (1999). Development of a PCR test for rapid diagnosis of contagious equine metritis. J Vet Med Sci. 61, 1287-1292.
Anzai T, Wada R, Okuda T, Aoki T, (2002). Evaluation of field application of PCR in the eradication of caontagious equine metritis from Japan. J Vet Med Sci. 64, 999- 1002.
Bleumink-Pluym NMC, Werdler MEB, Houwers DJ, Parlevliet JM, Colenbrander B, van der Zeijst BAM, (1994). Development and evaluation of PCR test for detection of Taylorella equigenitalis. J Clin Microbiol. 32, 893- 896.
Crowhurst RC, (1977). Genital infection in mares. Vet Rec. 100, 476.
Duquesne F, Pronost S, Laugier C, Petry S, (2007). Identification of Taylorella equigenitalis responsible for con- tagious equine metritis in equine genital swabs by direct polymerase chain reaction. Res Vet Sci. 82, 47-49.
Erdeğer J, Akan M, Altay G, Demirel M, (2002). Atlarda infertiliteye neden olan Taylorella equigenitalis’in bakteriyolojik ve serolojik tanısı. Vet J Ank Univ. 49, 39-44.
Jang SS, Donahue JM, Arata AB, Goris J, Hansen LM, Earley DL, Vandamme PA, Timoney PJ, Hirsh DC, (2001). Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus). Int J Syst Evol Microbiol. 51, 971-976.
Office International des Epizooties (OIE), (2008). Contagious equine metritis, Manual of diagnostic tests and vaccines for terrestrial animals, Chapter 2.5.2., p. 838-844.
Özgür NY, Eguchi M, Anzai T, Ikız S, Ilgaz A, (2001a). İnfertil ya da endometritisli kısraklarda rutin contagious equine metritis (CEM) taramalarında pasif hemaglutinasyon (PHA) testi ve ELISA’nın karşılaştırılması ve değerlendirilmesi. Turk J Vet Anim Sci. 25, 989- 994.
Özgür NY, Ikız S, Carıoglu B, Kılıcarslan R, Yılmaz H, Akay O, Ilgaz A, (2001b). Contagious equine metritis in Turkey: first isolation of Taylorella equigenitalis from mares. Vet Rec. 149, 120-122.
Watson ED, (1997). Swabbing protocols in screening for contagious equine metritis. Vet Rec. 140, 268-271. Geliş Tarihi / Received: 14.10.2009 Kabul Tarihi / Accepted: 16.11.2009 Yazışma adresi / Corresponding author Dr. H. Kaan Müştak Central Veterinary Control and Research Institute, Breeding Disease Laboratory, 06020, Etlik, Ankara, Turkey E-posta: [email protected]