SavedAdd your journal

Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2013 / Cilt: 24 Sayı: 1

Uterine Bacterial Profile of Germany Warm Blood Mares With Fertility Problems and Antimicrobial Susceptibility

Fertilite Sorunu Olan Alman Sıcakkanlı Irkı Kısrakların Uteruslarından İzole Edilen Bakteri Profili ve Antimikrobiyal Duyarlılıkları

Gülşen Goncagül(Veteriner Kontrol Merkez Araştırma Enstitüsü Müdürlüğü)
Kamil Seyrek İntaş(Veteriner Kontrol Merkez Araştırma Enstitüsü Müdürlüğü)
Pages
1–6
Publication Date
2013-12-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a conclusion, because antibiotic sensitivity of the bacteria leads to genital infections in mares are changes; treatments of the genital infections should be directed with the results of antibiogram and bacteriologic controls.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Endometritis is an important cause of subfertility with a high economic impact in mares.
📊
Key Findings & Data:Uterine microbiology and antimicrobial susceptibility was investigated in 342 mares with fertility problems in a retrospective study in Germany.
💡
Conclusion & Impact:As a conclusion, because antibiotic sensitivity of the bacteria leads to genital infections in mares are changes; treatments of the genital infections should be directed with the results of antibiogram and bacteriologic controls.
🔬Design:Araştırma Makalesi
📍Scope:307 samples
⚙️Variables:Etlik Veteriner Mikrobiyoloji Dergisi · Kısrak · Antimikrobiyal Duyarlılık

Abstract

Endometritis is an important cause of subfertility with a high economic impact in mares. Endometritis is mostly associated with a bacterial infection. Pathogen bacterias can cause fertility problems in mares. Furthermore, bacterial resistance to commonly used antibiotics leads to difficulties in the treatment of endometritis. Uterine microbiology and antimicrobial susceptibility was investigated in 342 mares with fertility problems in a retrospective study in Germany. Totally 307 samples (89.8%) were found culture positive. Amongst 307 bacterial isolates,12.7% were ß-hemolytic Streptococci and 20.8% Escherichia coli, hemolytic E.coli, coliform bacteria,12.3% γ-hemolytic Streptococci, 14.3% α-hemolytic Streptococci, 2.9% mold and 31.4% 27 some other bacteria isolates. Antibiogram test was performed to ß-hemolytic Streptococci and E.coli bacteria commonly resulted to fertility problems in mares. Antibiotic susceptibility of the isolates was determined to against 14 antibiotics. All ß-hemolytic Streptococci isolates were sensitive to florphenicol, ceftiofur, amoxicillin/clavulanic acid amoxicillin ve cefquinom. For E.coli 90% sensitivity rate was determined against enrof1oxacin, cefquinom and colistin. As a conclusion, because antibiotic sensitivity of the bacteria leads to genital infections in mares are changes; treatments of the genital infections should be directed with the results of antibiogram and bacteriologic controls.

Özet

Endometritis kısraklarda ciddi ekonomik kayıplara yol açan önemli kısırlık nedenidir. Endometritis, çoğunlukla bakteriyel enfeksiyonlar sonucunda ortaya çıkmaktadır. Patojen bakteriler, kısrakların fertilite problemlerine yol açabilmektedir. Ayrıca yaygın kullanılan antibiyotiklere karşı direnç gelişimi, endometritisin tedavisinde güçlüklere yol açmaktadır. Bu çalışmada Almanya’da fertilite problemi olan kısraklardan toplanan 342 adet uterus numunesi mikrobiyolojik etkenler ve antibiyotik duyarlılığı yönünden değerlendirildi. Toplam 307 adet (%89,8) numuneden bakteri izolasyonu yapıldı. Pozitif 307 numunenin, %12,7’si ß-hemolitik Streptokok %1,2 hemolitik Escherichia coli, %20,8‘i E.coli, %4,4’ü koliform bakteriler, %12,3’ü γ-hemolitik Streptokok, %14,3‘ü α-hemolitik Streptokok, %2,9‘u maya ve mantarlar, geri kalan %31,4‘ü ise 27 farklı bakteri izolasyonlarıdır. Kısrakların fertilitesini en sık etkileyen bakteriler olarak ß-hemolitik Streptokok ve E.coli etkenlerine karşı antibiyogram testi uygulandı. Pozitif numunelerde üreyen bakterilerin, 14 antibiyotiğe karşı duyarlılığı test edilmiştir. Tüm ß-hemolitik Streptokok izolatları florfenikol, seftiofur, amoksisilin/klavulanik asit, amoksisilin ve sefkuinom’a duyarlıydı. E.coli’ye karşı, enrofloksasin, sefkuinom ve kolistinnin %90‘nın üzerinde yüksek etki gösterdiği saptandı. Sonuç olarak, kısraklarda genital enfeksiyonlara yol açan bakterilerin antibiyotik duyarlılıkları değiştiği için tedaviler bakteriyolojik kontroller ve antibiyogram sonuçlarına göre yönlendirilmelidir.

🔖

Cite This Article

Goncagül, G., İntaş, K. S. (2013). Uterine Bacterial Profile of Germany Warm Blood Mares With Fertility Problems and Antimicrobial Susceptibility. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 24 Sayı: 1, 1–6. https://ajindex.com/articles/1166432

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (25)

  1. Albihn A, Baverud V, Magnusson U, (2003). Uterine microbiology and antimicrobial susceptibility in ısolated bacteria from mares with fertility problems. Acta Vet Scand. 44, 121-129.
  2. Amtsberg G, Karabisch P, (1975). Ergebnisse der bakteriologischen Zervixtupferuntersuchung von Warmblutstuten in den Jahren 1969-1973. Dtsch. Tierärztl Wschr. 82, 97-136.
  3. Atherton JG, Pitt TL, (1982). Types of Pseudomonas aeruginosa isolated from horses. Equine Vet J. 14, 329-332.
  4. Barrelet A, (1995). Laboratory aids to routine gynaecological management. Proc. Equine Stud. Medicine and AI Course British Equine Vet.Assoc. New-market, UK, p.52- 56.
  5. Blue MG, (1983). Mycotic invasion of the mare’s uterus.Vet Rec. 113, 131-133.
  6. Burleson MD, LeBlanc MM, Riddle WT, Hendricks KEM, (2010). Endometrial microbial isolates are associated with different ultrasonographic and endometrial cytology findings in Thoroughbred mares. Anim Reprod Sci Suppl. 121, 103.
  7. CLSI (2005). Performance Standards for Antimicrobial Susceptibility Testing,approved Standard M100-S15. Wayne, PA: Clinical and Laboratory Standards Institue.
  8. Cohen ML, (1992). Epidemiology of drug resistance: implications for a post-antimicrobial era. Science. 257, 1050- 1055.
  9. Ensink JM, Klingeren B, Houwers DJ, Klein WR, Vulto AG, (1993). In-vitro susceptibility to antimicrobial drugs of bacterial isolatesfrom horses in the Netherlands. Equine Vet J. 25, 309-313.
  10. Erdeğer J, Altay G, Akan M, Vural R, Dakman A, Çelebi M, (1999). Kısrakların genital organlarından izole edilen β hemolitik streptokok’ların identifikasyonu ve serogruplandırılması. Ankara Üniv Vet Fak Derg. 46, 69-76.
  11. Frontoso R, Carlo E, Pasolini MP,Meulen K, Pagnini U, Iovane G, Martino L, (2008). Retrospective study of bacterial isolates and their antimicrobial susceptibilities in equine uteri during fertility problems. Res Vet Sci. 84, 1-6.
  12. Graef EM, Decostere A, Devriese, LA, Haesebrouck F, (2004). Antibiotic resistance among fecal indicator bacteria from healthy individually owned and kennel dogs. Microb Drug Resist. 10, 65-69.
  13. Institut für Hygiene und Infektionskrankheiten der Tiere Justus-Liebeig-Universität Giessen (1996). Betr: Jahresbericht.
  14. Krüger A, (1976). Bakteriologische untersuchungen von Cervixtupfern von Stuten im Rahmen der zuchthygienischen überwachung der hannoverschen Warmblutzucht in den Jahren von 1971 bis 1975. Der praktische Tierarzt. 7, 411-416.
  15. Leblanc MM, (2003). Persistent mating induced endometritis in the mares: pathogenesis, diagnosis and treatment. In: Ball, B.A. (Ed.), Recent Advances in Equine Reproduction. International Veterinary Information Service, Ithaca, New York, USA (www.ivis.org).
  16. Neves AP, Keller A, Trein CR, Möller G, Jobim MIM, Castilho LFF, Cardoso MR, I.Leibold W, Zerbe H, Klug E, Gregory RM, Mattos RC, (2007). Use of leukocytes as treatment for endometritis in mares experimentally infected with Streptococcus equi subsp. Zooepidemicus. Anim Reprod Sci. 97, 314-322.
  17. Nielsen JM, (2005). Endometritis in the mare: A diagnostic study comparing cultures from swab and biopsy. Theriogenology. 64, 510-518.
  18. Oliver A, Canton R, Campos P, Baquero F, Blazquez J, (2000). High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science. 288, 1251-1254.
  19. Ricketts SW, Young A, Medici EB, (1993). Uterine clitorial cultures.In: Mckinnon, A.O., Voss, J.L. Equine Reproduction. Lea and Febringer, Philadelphia, 234-245.
  20. Schliesser T, Behtelsmann U, (1976). Bakteriologische Ergebnise bei der deckhygienischen Überwachung von Warmblutstuten in Hessen. Berl Münch Tierärztl Wschr. 89, 93-95.
  21. Seyrek İntaş K, Bostedt H, Herfen K, Seyrek İntaş D, (1997). Kısraklarda modifiye caslick metodu ile pneumovagina’nın operatif sağaltımı ve postoperatif sonuçlar. Vet Cer Derg. 3, 10-15.
  22. Seyrek İntaş K, Ülgen M, Mısırlıoğlu D, (1997). Bursa yöresinde kısraklada klinik, bakteriyolojik ve sitolojik muayeneler ile genital enfeksiyonların belirlenmesi. Ankara Univ Vet Fak Derd. 44, 31-38.
  23. Siu LK, (2002.). Antibiotics: action and resistance in gram-negative bacteria. J Microbiol Immunol. 35, 1-11.
  24. Sonnenschein B, Weiss R, (1978). Ergebnisse der bakteriologischen zervixtupferuntersuchung von Warmblut und traberstuten in den jahren 1974-1977. Berl Münch Tierärztl Wschr. 91, 123-128.
  25. Zonturlu AK, Kaçar C, (2004). Kısraklarda endometritisin tanı ve tedavi yöntemleri. Kafkas Univ Vet Fak. 10, 131-134.