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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2026 / Cilt: 36 Sayı: 1

Seroprevalence of Brucella spp. in Cattle in the Central Black Sea Region and Investigation of the Effect of Brucellosis on Oxidative Stress Parameters

Orta Karadeniz Bölgesindeki Sığırlarda Brucella spp.’nin Seroprevalansı ve Brusellozun Oksidatif Stres Parametreleri Üzerindeki Etkisinin İncelenmesi

Onur Taş(Samsun Veteriner Kontrol Enstitü Müdürlüğü)0000-0003-0577-4024
Büşra Şahin(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0002-5245-478X
Yunus Kılıçoğlu(Samsun Veteriner Kontrol Enstitüsü Müdürlüğü)0000-0002-6788-9284
Sena Çenesiz(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0002-3544-503X
Adem Aranci(Samsun Veteriner Kontrol Enstitüsü Müdürlüğü)0009-0004-3906-8368
Ali Ertekin(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0002-6299-9716
Neslihan Ormancı(Samsun Veteriner Kontrol Enstitüsü Müdürlüğü)0000-0001-7645-3792
Rahşan Koç Akpınar(Samsun Veteriner Kontrol Enstitüsü Müdürlüğü)0000-0003-0075-9247
Mehmet Çitil(ERCİYES ÜNİVERSİTESİ)0000-0001-9839-7533
Pages
73–81
Publication Date
2025-07-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it was determined that the oxidant-antioxidant balance was disturbed in cattle infected with Brucella and oxidative stress occurred accordingly.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to determine the seroprevalence of Brucella spp. in cattle in the Central Black Sea Region and to investigate the effect of brucellosis on oxidative stress parameters such as Total thiol, Native thiol, Disulphide, Native thiol/Total thiol, Disulfide/Native thiol, Disulphide/Total thiol, MDA levels and ADA.
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Key Findings & Data:According to our study results, 155 (1.42%) serum samples were positive for Brucella spp. while 10727 (98.58%) serum samples were negative.
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Conclusion & Impact:In conclusion, it was determined that the oxidant-antioxidant balance was disturbed in cattle infected with Brucella and oxidative stress occurred accordingly.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Brucella · Oksidatif stres · Serum

Abstract

The aim of this study was to determine the seroprevalence of Brucella spp. in cattle in the Central Black Sea Region and to investigate the effect of brucellosis on oxidative stress parameters such as Total thiol, Native thiol, Disulphide, Native thiol/Total thiol, Disulfide/Native thiol, Disulphide/Total thiol, MDA levels and ADA. According to our study results, 155 (1.42%) serum samples were positive for Brucella spp. while 10727 (98.58%) serum samples were negative. Antibody titres of 99.35% of the positive serum samples were 1/40 and above. In order to analyse the effect of brucellosis on oxidative stress parameters, 23 positive serum samples and 23 negative serum samples were used as control group. In biochemical analyses; Total thiol, Native thiol, Disulphide, MDA and ADA values in the Brucella group were significantly higher than the values in the control group. Disulfide/Native thiol, Disulphide/Total thiol values in the Brucella group did not show a significant change when compared with the values in the control group. In addition, Native thiol/Total thiol value in the Brucella group was found to be significantly lower than the value in the control group. In conclusion, it was determined that the oxidant-antioxidant balance was disturbed in cattle infected with Brucella and oxidative stress occurred accordingly. These findings suggest that oxidative stress has the potential to be used as a biomarker in the diagnosis and treatment of Brucella disease. In addition, according to the seroprevalence data obtained in the study, Brucella infection continues to threaten public health and animal health in the Central Black Sea Region.

Özet

Bu çalışmanın amacı Orta Karadeniz Bölgesindeki sığırlarda Brucella spp. seroprevalansının belirlenmesi ve brusellozun oksidatif stres parametrelerinden Total thiol, Native thiol, Disulphide, Native thiol/Total thiol, Disulfide/Native thiol, Disulphide/Total thiol, MDA düzeyleri ve ADA gibi oksidatif stress parametreleri üzerindeki etkisinin incelenmesidir. Çalışma sonuçlarımıza göre 155 adet (%1,42) serum örneği Brucella spp. açısından pozitif olarak tespit edilirken 10727 adet (%98,58) serum örneği negatif olarak tespit edildi. Pozitif olarak tespit edilen serum örneklerinin %99,35’inin antikor titresi 1/40 ve üzeri bulundu. Brusellozun oksidatif stres parametreleri üzerindeki etkisini incelemek amacıyla pozitif örneklerden 23 adet ve kontrol grubunu oluşturmak üzere 23 adet negatif serum örneği kullanıldı. Biyokimyasal analizlerde; Brucella gruptaki Total thiol, Native thiol, Disulphide , MDA ve ADA değerleri, kontrol gruptaki değerlere göre anlamlı derecede yüksek bulunmuştur. Brucella grubunda ki Disulfide/Native thiol , Disulphide/Total thiol değerleri, kontrol grubunda ki değerleri ile karşılaştırıldıklarında anlamlı düzeyde bir değişiklik bulunmamıştır. Ayrıca Brucella grubunda ki Native thiol/Total thiol değeri ise kontrol grubunda ki değerine göre anlamlı düzeyde düşük bulunmuştur. Sonuç olarak, Brucella ile enfekte sığırlarda oksidan-antioksidan dengesinin bozulduğu ve buna bağlı olarak oksidatif stresin ortaya çıktığı belirlendi. Bu bulgular, oksidatif stresin, Brucella hastalığı tanı ve tedavisinde biyobelirteç olarak kullanım potansiyeline sahip olduğunu düşündürmektedir. Ayrıca çalışmada elde edilen seroprevalans verilerine göre Brucella enfeksiyonun Orta Karadeniz Bölgesinde halk sağlığını ve hayvan sağlığını tehdit etmeye devam ettiğini göstermektedir.

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Cite This Article

Taş, O., Şahin, B., Kılıçoğlu, Y., Çenesiz, S., Aranci, A., Ertekin, A., Ormancı, N., Akpınar, R. K., Çitil, M. (2025). Seroprevalence of Brucella spp. in Cattle in the Central Black Sea Region and Investigation of the Effect of Brucellosis on Oxidative Stress Parameters. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 36 Sayı: 1, 73–81. https://doi.org/10.35864/evmd.1592424

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