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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2025 / Cilt: 36 Sayı: 2

Molecular investigation of dogs' tick-borne infections in Karaman province of Türkiye

Türkiye’nin Karaman ilinde köpeklerde kene kaynaklı enfeksiyonların moleküler incelenmesi

Ayşe Coşkun(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ)0000-0003-4538-8873
Mehmet Fatih Aydın(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ FAKÜLTESİ)0000-0002-8325-4887
Pages
123–134
Publication Date
2026-01-13
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Following 16 samples that tested positive for the Hepatozoan genus, it was found that all of them were Hepatozoon canis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Tick-transmitted diseases in dogs are growing more significant in Türkiye and around the world.
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Key Findings & Data:Polymerase chain reaction (PCR) and DNA sequence analysis techniques were used to analyze the isolated DNAs in terms of the 16S rRNA gene of Anaplasma, Ehrlichia, and hemotropic Mycoplasma species, as well as the 18S rRNA gene of Babesia and Hepatozoon species.
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Conclusion & Impact:Following 16 samples that tested positive for the Hepatozoan genus, it was found that all of them were Hepatozoon canis.
🔬Design:Araştırma Makalesi
📍Scope:16 samples
⚙️Variables:Anaplasma · Babesia · Ehrlichia

Abstract

Tick-transmitted diseases in dogs are growing more significant in Türkiye and around the world. In areas with tropical and subtropical climates, Hepatozoon, Babesia, Anaplasma, Ehrlichia, and hemotropic Mycoplasma species are among the pathogens that produce both clinical and subclinical illnesses. In this work, the prevalence of Hepatozoon spp., Babesia spp., Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Anaplasma platys, and Ehrlichia canis species in dogs in the southern part of Türkiye’s Central Anatolia Region was examined using molecular techniques. Polymerase chain reaction (PCR) and DNA sequence analysis techniques were used to analyze the isolated DNAs in terms of the 16S rRNA gene of Anaplasma, Ehrlichia, and hemotropic Mycoplasma species, as well as the 18S rRNA gene of Babesia and Hepatozoon species. Of the 140 extracted DNA samples, 32 (22.85%) Hepatozoon spp., 36 (25.71%) M. haemocanis, and 12 (8.57%) contained Ca. M. haematoparvum. Following 16 samples that tested positive for the Hepatozoan genus, it was found that all of them were Hepatozoon canis. Two of the samples were 100% comparable to the H. canis sequences found in GenBank, while the remaining samples were between 98.98% and 99.84% similar. Sequencing of two representative M. haemocanis and one Ca. M. haematoparvum sample revealed that the Ca. M. haematoparvum isolates were 99.19–99.60% similar to the Ca. M. haematoparvum isolates listed in GenBank, but the M. haemocanis samples were 100% identical to other M. haemocanis isolates. Dogs older than one year had considerably higher hemoplasma positivity, and both hemoplasma and Ca. M. haematoparvum positivity rose significantly with mean age (p<0.05). These findings offer the first comprehensive details regarding tick-borne illnesses in dogs in Türkiye's southern Central Anatolia Region.

Özet

Köpeklerde kene ile bulaşan hastalıklar Türkiye'de ve dünyada giderek daha önemli hale gelmektedir. Tropikal ve subtropikal iklime sahip bölgelerde, Hepatozoon, Babesia, Anaplasma, Ehrlichia ve hemotropik Mycoplasma türleri hem klinik hem de subklinik hastalıklara neden olan patojenler arasındadır. Bu çalışmada, Türkiye'nin İç Anadolu Bölgesi'nin güney kesimindeki köpeklerde Hepatozoon spp., Babesia spp., Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Anaplasma platys ve Ehrlichia canis türlerinin prevalansı moleküler teknikler kullanılarak incelenmiştir. İzole edilen DNA'ların Anaplasma, Ehrlichia ve hemotropik Mycoplasma türlerinin 16S rRNA geninin yanı sıra Babesia ve Hepatozoon türlerinin 18S rRNA geni açısından analiz edilmesi için polimeraz zincir reaksiyonu (PCR) ve DNA dizi analizi teknikleri kullanılmıştır. Ekstrakte edilen 140 DNA örneğinin 32'si (%22,85) Hepatozoon spp., 36'sı (%25,71) M. haemocanis ve 12'si (%8,57) Ca içermektedir. M. haematoparvum içermektedir. Hepatozoan cinsi için pozitif test edilen 16 örneğin ardından, bunların hepsinin Hepatozoon canis olduğu tespit edilmiştir. Örneklerden ikisi GenBank'ta bulunan H. canis dizileriyle %100, geri kalan örnekler %98,98 ile %99,84 arasında benzerlik göstermiştir. İki temsili M. haemocanis ve bir Ca. M. haematoparvum örneğinin dizilenmesi ile, Ca. M. haematoparvum izolatlarının %99,19-99,60 oranında GenBank'ta listelenen Ca. M. haematoparvum izolatları ile, M. haemocanis örneklerinin ise diğer M. haemocanis izolatları ile %100 aynı olduğu ortaya konmuştur. Bir yaşından büyük köpeklerde hemoplazma pozitifliği oldukça yüksekti ve hem hemoplazma hem de Ca. M. haematoparvum pozitifliği ortalama yaşla birlikte önemli ölçüde artmıştır (p<0.05). Bu bulgular, Türkiye'nin güney İç Anadolu Bölgesi'ndeki köpeklerde kene kaynaklı hastalıklara ilişkin ilk kapsamlı ayrıntıları sunmaktadır.

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Cite This Article

Coşkun, A., Aydın, M. F. (2026). Molecular investigation of dogs' tick-borne infections in Karaman province of Türkiye. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 36 Sayı: 2, 123–134. https://doi.org/10.35864/evmd.1572916

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References (72)

  1. Aktas M, Ozubek S, Sayın Ipek DN. (2013). Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus. Parasitol Res, 112, 2381–2385. doi: 10.1007/s00436-013-3403-6
  2. Aktas M. (2014). A survey of ixodid tick species and molecular identification of tick-borne pathogens. Vet Parasitol, 200(3-4), 276-283. doi:10.1016/j.vetpar.2013.12.008
  3. Aktas M, Ozubek S, Altay K, Balkaya İ, Utuk AE, Kırbas A, Simsek S, Dumanlı N. (2015a). A moleculer and parasitological survey of Hepatozoon canis in domestic dogs in Turkey. Vet Parasitol, 209(3-4), 264-267. doi: 10.1016/j.vetpar.2015.02.015
  4. Aktas M, Ozubek S, Altay K, Ipek ND, Balkaya İ, Utuk AE, Kırbas A, Simsek S, Dumanlı N. (2015b). Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey. Parasit Vectors, 8(157). doi:10.1186/s13071-015-0763-z
  5. Aktas M, Ozubek S. (2017a). A survey of canine haemoprotozoan parasites from Turkey, including molecular evidence of an unnamed Babesia. Comp Immunol Microbiol Infect Dis, 52, 36-42. doi:10.1016/jivetmic.06.004
  6. Aktas M, Ozubek S. (2017b). Molecular survey of haemoplasmas in shelter dogs and associations with Rhipicephalus sanguineus sensu lato. Med Vet Entomol, 31(4), 457-461. doi:10.1111/mve.12244
  7. Aktas M, Ozubek S. (2018). A molecular survey of hemoplasmas in domestic dogs from Turkey, Vet Microbiol, 221, 94-97. doi:10.1186/s13071-015-0763-z
  8. Altay K, Aydin MF, Aytmirzakizi A, Jumakanova Z, Cunusova A, Dumanli N. (2019). Molecular survey of hepatozoonosis in natural infected dogs: First detection and molecular characterisation of Hepatozoon canis in Kyrgyzstan. Kafkas Univ Vet Fak Derg, 25(1), 77-81. 2019. doi: 10.9775/ kvfd.2018.20352
  9. Altay K, Aydın MF, Aytmirzakizi A, Jumakanova Z, Cunusova A, Dumanlı N. (2020) First molecular evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in asymptomatic shelter dogs in Kyrgyzstan. Kafkas Univ Vet Fak Derg, 26(1), 143-146. doi: 10.9775/kvfd.2019.22196
  10. Altay K, Erol U, Sahin OF, Aydin MF, Aytmirzakizi A, Dumanli N. (2023). First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan. Pathogens, 12(8): 1046. doi: 10.3390/pathogens12081046.
  11. Anonim, Adrese Dayalı Nüfus Kayıt Sistemi Sonuçları. https://biruni.tuik.gov.tr/medas/?kn=95&locale=tr - (Erişim Tarihi: 27.07.2021).
  12. Aquino LC, Kamanid J, Harunae AM, Paludoa GR, Hicksb CA, Helps CR, Tasker S. (2016). Analysis of risk factors and prevalence of haemoplasma infection in dogs. Vet Parasitol, 221, 111-117. doi:10.1016/j.vetpar.2016.03.014
  13. Aydin MF, Aktas M, Dumanli N. (2013). Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res, 112, 2817-2824. doi: 10.1007/s00436-013-3452-x
  14. Aydin MF, Sevinc F, Sevinc M. (2015). Molecular detection and characterizasyon of Hepatozoon spp. in dogs from the central part of Turkey. Ticks Tick Borne Dis, 6(3), 388-392. doi: 10.1016/j.ttbdis.03.004
  15. Aysul N, Ural K, Ulutaş B, Eren H, Karagenc T. (2013). First detection and molecular identification of Babesia gibsoni in two dogs from the Aydın province of Turkey. Turk J Vet Anim Sci, 37(2), 226-229. doi:10.3906/vet-1201-45
  16. Baneth G, Vincent-Johson N. (2005). Hepatozoonosis. In: Shaw, E.S., Day, M.J.(Eds). Artrhropod-borne infectious diseases of the dogs and cats. Lippincott Williams & Wilkins.
  17. Baneth G. (2011). Perspectives on canine and feline hepatozoonosis. Vet Parasitol, 181, 3-11. doi: 10.1016/j.vetpar.2011.04.015
  18. Barker EN, Tasker S, Day MJ, Warman SM, Woolley K, Birtles R, Georges KC, Ezeokoli CD, Newaj-Fyzul A, Campbell MD, Sparagano OA, Cleaveland S, Helps CR. (2010). Development and use of real-time PCR to detect and quantify Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in dogs. Vet Microbiol, 140, 167-170. doi: 10.1016/j.vetmic.2009.07.006.ü
  19. Barker EN, Langton DA, Helps CR, Brown G, Malik R, Shaw SE, Tasker S. (2012). Haemoparasites of free-roaming dogs associated with several remote Aboriginal communities in Australia. BMC Vet Res, 8, 55. doi:10.1186/1746-6148-8-55
  20. Beck R, Vojta L, Mrljak V, Marinculic A, Beck A, Zˇivicˇnjak T, Caccio SM. (2009). Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. Int J Parasitol, 39, 843-848. doi: 10.1016/j.ijpara.2008.12.005
  21. Bolukbas CS, Pekmezci D, Gurler AT, Pekmezci GZ, Guzel M, Acici M, Umur S. (2016). Molecular survey of Hepatozoon canis in dogs from Samsun Province of Northern part of Turkey. Etlik Vet Mikrobiyol Derg, 27(2), 104-107. doi:10.35864/evmd.514510
  22. Cacciò SM, Antunovic B, Moretti A, Mangili V, Marinculic A, Baric RR, Slemenda SB, Pieniazek NJ. (2002). Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs. Vet Parasitol, 106(4), 285-292. doi:10.1016/s0304-4017(02)00112-7
  23. Casati S, Sager H, Gern L, Piffaretti JC. (2006). Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med, 13(1), 65-70.
  24. Ceylan O, Sevinc F. (2020). Endemic instability of ovine babesiosis in Turkey: A country-wide sero-epidemiological study. Vet Parasitol, 278. doi: 10.1016/j.vetpar.2020.109034
  25. Chomel B. (2011). Tick-borne infections in dogs-An emerging infectious threat. Vet Parasitol, 179, 294-301. doi:10.1016/j.vetpar.2011.03.040
  26. Compton SM, Maggi RG, Breitschwerdt EB. (2012). Candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis infections in dogs from the United States. Comp Immunol Microbiol Infect Dis, 35, 557-562. doi:10.1016/j.cimid.2012.06.004
  27. Dumanli N, Altay K, Aktas M. (2016). Keneler ve kenelerle taşınan hastalıklar. Manas J Agric Vet Life Sci, 6(2), 45-54.
  28. Dumler JS, Barbet AF, Bekker CPJ, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR. (2001). Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with
  29. Neorickettsia, descriptions of six new species combinations and designation of Ehrlichiaequi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol, 51, 2145-2165. doi:10.1099/00207713-51-6-2145
  30. Erol U, Altay K, Atas AD, Ozkan E, Sahin OF. (2021a). Molecular Prevalence of Canine Hepatozoonosis in Owned-Dogs in Central Part of Turkey. Isr J Vet Med, 76(2), 71-76.
  31. Erol U, Altay K, Atas AD, Sahi̇n OF, Urhan OF. (2021b). First molecular detection of Canine Hemoplasmas in Sivas province in central part of Turkey. Etlik Vet Mikrobiyol Derg, 32(1), 27-32. https://doi.org/10.35864/evmd.903166
  32. Gettings JR, Lopez JE, Krishnavahjala A, Armstrong BA, Thompson AT, Yabsley MJ. (2019). Antibodies to Borrelia turicatae in Experimentally Infected Dogs Cross-React with Borrelia burgdorferi Serologic Assays. J Clin Microbiol, 57(9):e00628-19. doi: 10.1128/JCM.00628-19
  33. Gokce E, Kırmızıgül AH, Tasci GT, Uzlu E, Gündüz N, Vatansever Z. (2013). Türkiye’de köpeklerde Babesia canis canisin klinik ve parazitolojik olarak ilk tesbiti. Kafkas Univ Vet Fak Derg, 19(4), 717-720. doi: 10.9775/kvfd.2013.7598
  34. Guo H, Sevinc F, Ceylan O, Sevinc M, Ince E, Gao Y, Moumouni PFA, Liu M, Efstratiou A, Wang G, Cao S, Zhou M, Jirapattharasate C, Ringo AE, Zheng W, Xuan X. (2017). A PCR survey of vector-borne pathogens in different dog populations from Turkey. Acta Parasitol, 62(3), 533-540. doi: 10.1515/ap-2017-0064
  35. Guven E, Avcioglu H, Cengiz S, Hayirli A. (2017). Vector-Borne Pathogens in Stray Dogs in Northeastern Turkey. Vector Borne Zoonotic Dis, 17(8), 610-617. doi:10.1515 / ap-2017-0064
  36. Gulanber A, Gorenflot A, Schetters TP, Carcy B. (2006). First molecular diagnosis of Babesia vogeli in domestic dogs from Turkey. Vet Parasitol, 139(1-3), 224-230. doi: 10.1515 / ap-2017-0064
  37. Harrus S, Waner T, Bjöerdorff A, Shaw S. (2005). Ehrlichiosis and anaplasmosis. In: Shaw, E.S., Day, M.J.(Eds). Artrhropod-borne infectious diseases of the dogs and cats. Lippincott Williams & Wilkins.
  38. Inci A, Yildirim A, Duzlu O, Doganay M, Aksoy S. (2016). Tick-borne diseases in Turkey: A review based on one health perspective. PLoS Negl Trop Dis, 10:e0005021. doi: 10.1371/journal.pntd.0005021
  39. Inokuma H, Okuda M, Ohno K, Shimoda K, Onishi T. (2002). Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Vet Parasitol, 106, 265-271. doi:10.1016/S0304-4017(02)00065-1
  40. Irwin PJ. (2009). Canine babesiosis: from molecular taxonomy to control. Parasit Vectors, 2, 51-54. doi:10.1186/1756-3305-2-S1-S4
  41. Karagenc T, Hosgor M, Bilgic HB, Pasa S, Kirli G, Eren H. (2005). The determination of the prevalance of E. canis, A. phagocytophila, A. platys with nested PCR in dogs in the Aegean Region. İzmir, Turkey: 14th Nationatal Parasitology Congress, 18-25.
  42. Karagenc TI, Pasa S, Kirli G, Hosgor M, Bilgic HB, Ozon YH, Atasoy A, Eren H. (2006). A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey. Vet Parasitol, 135, 113-119. doi:10.1016/j.vetpar.2005.08.007
  43. Katoh K, Standley DM. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol, 30(4), 772-780. doi: 10.1093/molbev/mst01
  44. Kenny MJ, Shaw SE, Beugnet F, Tasker S. (2004). Demonstration of two distinct hemotropic mycoplasmas in French dogs. J Clin Microbiol, 42, 5397-5399. doi:10.1128/JCM.42.11.5397-5399.2004
  45. Kjemtrup AM, Wainwright K, Miller M, Penzhorn BL, Carreno RA. (2006). Babesia conradae, sp. nov., a small canine Babesia identified in California. Vet Parasitol, 138, 103-111. doi:10.1016/j.vetpar.2006.01.044
  46. Little SE, Allen KE, Johnson EM, Panciera RJ, Reichard MV, Ewing SA. (2009). New developments in canine hepatozoonosis in North America: a review’’, Parasit Vectors. 2, 1-5. doi:10.1186/1756-3305-2-S1-S5
  47. Little SE. (2010). Ehrlichiosis and Anaplasmosis in Dogs and cats. Veterinary Clinics of North America. J Small Anim Pract, 40, 1121-1140. doi: 10.1016/j.cvsm.2010.07.004
  48. Matijatko V, Torti M, Schetters TP. (2012). Canine babesiosis in Europe: how many diseases? Trends Parasitol, 28(3), 99-105. doi:10.1016/j.pt.2011.11.003
  49. Messick JB. (2004). Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet Clin Pathol, 33, 1-13. doi:10.1111/j.1939-165x.2004.tb00342.x
  50. Motoi Y, Satoh H, Inokuma H, Kiyuuna T, Muramatsu Y, Ueno H, Morita C. (2001). First detection of Ehrlichia platys in dogs and ticks in Okinawa, Japan. Microbiol Immunol, 45 (1), 89-91.
  51. Murphy GL, Ewing SA, Whitworth LC, Fox JC, Kocan AA. (1998). A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet Parasitol, 79(4), 325-339. doi:10.1016/s0304-4017(98)00179-4
  52. Novacco M, Meli ML, Gentilini F, Marsilio F, Ceci C, Pennisi MG, Lombardo G, Lloret A, Santos L, Carrapic OT, Willi B, Wolf G, Lutz H, Hofmann-Lehmann R. (2010). Prevalence and geographical distribution of canine hemotropic mycoplasma infections in Mediterranean countries and analysis of risk factors for infection. Vet Microbiol, 142, 276-284. doi:10.1016/j.vetmic.2009.09.069
  53. Orkun Ö, Koc N, Sürsal N, Cakmak A, Nalbantoğlu S, Karaer Z. (2018) Molecular characterization of tick-borne blood protozoa in stray dogs from Central Anatolia Region of Turkey with a high-rate Hepatozoon infection. Kafkas Univ Vet Fak Derg, 24(2), 227-232. doi:10.9775/kvfd.2017.18678
  54. Ozubek S, Ipek DNS, Aktas M. (2018). A Molecular Survey of Rickettsias in Shelter Dogs and Distribution of Rhipicephalus sanguineus (Acari: Ixodidae) sensu lato in Southeast Turkey. J Med Entomol, 55(2), 459-463. doi:10.1093/jme/tjx213
  55. Pasa S, Kiral F, Karagenc T, Atasoy A, Seyrek K. (2009). Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turk J Vet Anim Sci, 33(4), 289-95. doi:10.3906/vet-0801-11
  56. Roura X, Peters IR, Altet L, Tabar MD, Barker EN, Planellas M, Helps CR, Francino O, Shaw SE, Tasker S. (2010). Prevalence of hemotropic mycoplasmas in healthy and unhealthy cats and dogs in Spain. J Vet Diagn Inves, 22, 270-274. doi:10.1177/104063871002200219
  57. Sari B, Tasci GT, Kilic Y. (2013). Seroprevalence of Dirofilaria immitis, Ehrlichia canis and Borrelia burgdorferi in dogs in Iğdır province, Turkey. Kafkas Univ Vet Fak Derg, 19(5), 735-9. doi:10.9775/kvfd.2012.8466
  58. Sasaki M, Ohta K, Matsuu A, Hirata H, Ikadai H, Oyamada T. (2008). A molecular survey of Mycoplasma haemocanis in dogs and foxes in Aomori Prefecture. J Protozool Res, 18, 57-60. doi:10.32268/jprotozoolres.18.2_57
  59. Shaw ES, Day MJ. (2005). Artrhropod-borne infectious diseases of the dogs and cats. Lippincott Williams & Wilkins.
  60. Skotarczak B. (2003). Canine ehrlichiosis. Ann Agric Environ Med, 10(2), 137-141.
  61. Soares RL, Echeverria JT, Pazzuti G, Cleveland KHP, Babo-Terra VJ, Friozi E, Ramos CAN. (2016). Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil. Rev Bras Parasitol Vet, 25, 359-363. doi:10.1590/S1984-29612016043
  62. Solano-Gallego L, Baneth G. (2011). Babesiosis in dogs and cats-Expanding parasitological and clinical spectra. Vet Parasitol, 181, 48-60. doi:10.1016/j.vetpar.2011.04.023
  63. Sykes JE, Ball LM, Bailiff NL, Fry MM. (2005). 'Candidatus Mycoplasma haematoparvum', a novel small haemotropic mycoplasma from a dog. Int J Syst Evol Microbiol, 55(Pt 1), 27-30. doi: 10.1099/ijs.0.02989-0
  64. Tennant KV, Barker EN, Polizopoulou Z, Helps CR, Tasker S. (2011). Real-time quantitative polymerase chain reaction detection of haemoplasmas in healthy and unhealthy dogs from Central Macedonia, Greece. J Small Anim Pract, 52, 645-649. doi:10.1111/j.1748-5827.2011.01126.x
  65. Torkan S, Aldavood SJ, Sekhavatmandi A, Moshkelani S. (2014). Detection of haemotropic Mycoplasma (Haemobartonella) using multiplex PCR and its relationship with epidemiological factors in dogs. Comp Clin Path, 23, 669-672. doi:10.1007/s00580-012-1668-2
  66. Tunc HÖ, Aktas MS. (2016). Türkiye’de Köpeklere Kene Aracılığıyla Bulaşan Hastalıklar. Erciyes Üniv Vet Fak Derg, 13(3), 223-230.
  67. Ulutas B, Bayramli G, Karagenc T. (2007). First case of anaplasma (ehrlichia) platys infection in a dog in Turkey. Turk J Vet Anim Sci, 31(4), 279-282.
  68. Ural K, Gültekin M, Atasoy A, Ulutas B. (2014). Spatial distribution of vector borne disease agents in dogs in Aegean region, Turkey. Rev mvz Cordoba, 19(2), 4086-4098. Valle Sde F, Messick JB, Dos Santos AP, Kreutz LC, Duda NC, Machado G, Corbellini LG, Biondo AW, González FH. (2014). Identification, occurrence and clinical findings of canine hemoplasmas in southern Brazil. Comp Immunol Microbiol Infect Dis, 37(4), 259-265.
  69. Vieira RF, Vidotto O, Vieira TS, Guimaraes AM, Santos AP, Nascimento NC, Santos NJ, Martins TF, Labruna MB, Marcondes M, Biondo AW, Messick JB. (2015). Molecular investigation of hemotropic mycoplasmas in human beings, dogs and horses in a rural settlement in southern Brazil. Rev Inst Med Trop Sao Paulo, 57, 353-357.
  70. Vojta L, Mrljak V, Curkovic S, Zˇivicˇnjak T, Marinculic A, Beck R. (2009). Molecular epizootiology of canine hepatozoonosis in Croatia. Int J Parasitol, 39, 1129-1136. doi:10.1016/j.ijpara.2009.02.007 Zhang Z, Schwartz S, Wagner L, Miller W. (2000). A greedy algorithm for aligning DNA sequences. J Comput Biol, 7(1-2): 203-214. doi: 10.1089/10665270050081478
  71. Aktas M, Ozubek S. (2018). A molecular survey of hemoplasmas in domestic dogs from Turkey, Vet Microbiol, 221, 94-97. doi:10.1186/s13071-015-0763-z
  72. Anonim, Adrese Dayalı Nüfus Kayıt Sistemi Sonuçları. https://biruni.tuik.gov.tr/medas/?kn=95&locale=tr - (Erişim Tarihi: 27.07.2021).