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Journals / Türkiye Entomoloji Dergisi / 2026 / Cilt: 49 Sayı: 2

Determination of tannin activity and Paenibacillus alvei (Cheshire & Cheyne) Ash et al. (Bacillii: Paenibacillaceae) on the biocontrol of tannin-tolerant Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) larvae

Tanen toleranslı Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) larvalarının biyolojik mücadelesinde Paenibacillus alvei (Cheshire & Cheyne) Ash et al. (Bacillii: Paenibacillaceae) ve tanen aktivitesinin belirlenmesi

Nurver Altun(Recep Tayyip Erdoğan Üniversitesi)0000-0002-2657-9263
Emel Topçu(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0000-0003-0700-3426
Leyla Kılcı(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0009-0009-9160-4662
Şengül Alpay Karaoğlu(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0000-0003-1047-8350
Özlem Faiz(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0000-0003-2447-0763
Pages
213–227
Publication Date
2025-06-25
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The diet containing 5% tannic acid had the lowest mortality rate.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Tannins are among the most abundant secondary metabolites synthesized by plants.
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Key Findings & Data:Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) is a critical forest pest.
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Conclusion & Impact:The diet containing 5% tannic acid had the lowest mortality rate.
🔬Design:Araştırma Makalesi
📍Scope:2022 Verileri
⚙️Variables:Agelastica alni · antioksidan sistem · biyolojik mücadele

Abstract

Tannins are among the most abundant secondary metabolites synthesized by plants. Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) is a critical forest pest. This study investigated the effect of Paenibacillus alvei (Cheshire & Cheyne) Ash et al. (Bacillii: Paenibacillaceae) and tannins against A. alni larvae. The larvae were collected from the Çayeli district of Rize province in 2022. In the feeding experiments, artificial diets containing 1.25%, 2.5% and 5% tannins were prepared. 100 and 200 µl of P. alvei were applied to the infected groups. Nutritional indices, pupal masses, phenoloxidase activities, antioxidant enzyme activities and mortality rates of larvae fed with different diets were studied. Relative consumption rate (RCR) increased with tannin concentration in all groups. Relative growth rate (RGR) increased with rising tannin concentrations across all groups. In the infected groups, the increase in tannin concentration caused a decrease in developmental time. While superoxide dismutase and phenoloxidase activities of uninfected larvae decreased with tannin concentration, catalase and glutathione peroxidase activities of larvae increased. In infected larvae, catalase activity decreased with increasing tannin concentration. The dose of P. alvei caused an increase in superoxide dismutase and phenoloxidase activities, but did not affect catalase and glutathione peroxidase activities. The diet containing 5% tannic acid had the lowest mortality rate.

Özet

Tanenler, bitkiler tarafından sentezlenen en bol ikincil metabolitler arasındadır. Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) önemli bir orman zararlısıdır. Bu çalışmada, Paenibacillus alvei (Cheshire & Cheyne) Ash et al. (Bacillii: Paenibacillaceae) ve tanenlerin A. alni larvaları üzerindeki etkisi araştırılmıştır. Larvalar 2022 yılında Rize ili Çayeli ilçesinden toplanmıştır. Besleme deneylerinde, %1.25, %2.5 ve %5 tanen içeren yapay diyetler hazırlanmıştır. Enfekte gruplara 100 ve 200 µl P. alvei uygulanmıştır. Farklı diyetlerle beslenen larvaların beslenme indeksleri, pupal kütleleri, fenoloksidaz aktiviteleri, antioksidan enzim aktiviteleri ve ölüm oranları incelenmiştir. Nisbi tüketim oranı (RCR), tüm gruplarda tanen konsantrasyonu ile artmıştır. Nisbi büyüme oranı (RGR), tüm gruplarda tanen konsantrasyonunun artmasıyla yükselmiştir. Enfekte gruplarda tanen konsantrasyonundaki artış, gelişim süresinde azalmaya neden olmuştur. Enfekte olmayan larvalarda süperoksit dismutaz ve fenoloksidaz aktiviteleri tanen konsantrasyonu ile azalırken, katalaz ve glutatyon peroksidaz aktiviteleri artmıştır. Enfekte larvalarda ise tanen konsantrasyonundaki artış katalaz aktivitesinin azalmasına neden olmuştur. P. alvei dozu, süperoksit dismutaz ve fenoloksidaz aktivitelerinde artışa yol açarken, katalaz ve glutatyon peroksidaz aktivitelerini etkilememiştir. %5 tanik asit içeren diyet, en düşük ölüm oranına sahip olmuştur.

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Cite This Article

Altun, N., Topçu, E., Kılcı, L., Karaoğlu, Ş. A., Faiz, Ö. (2025). Determination of tannin activity and Paenibacillus alvei (Cheshire & Cheyne) Ash et al. (Bacillii: Paenibacillaceae) on the biocontrol of tannin-tolerant Agelastica alni L., 1758 (Coleoptera: Chrysomelidae) larvae. Türkiye Entomoloji Dergisi, Cilt: 49 Sayı: 2, 213–227. https://doi.org/10.16970/entoted.1661578

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