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Journals / Türkiye Entomoloji Dergisi / 2025 / Cilt: 49 Sayı: 4

Evaluation of the insecticidal activity of some Turkish entomopathogenic fungal isolates against second larval stage of Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)

Bazı Türkiye entomopatojen fungus izolatlarının Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)'nın ikinci larva dönemine karşı insektisidal etkisinin değerlendirilmesi

Neslihan Kara(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0002-6607-1229
Dürdane Yanar(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0003-2517-1538
Pages
401–412
Publication Date
2026-01-06
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Promising results were obtained in the control of tomato leaf miner using the GOPT-DYYLD and BBE2 isolates.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the efficacy of two local isolates of the entomopathogenic fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. (GOPT-DYYLD, BBE2) (Hypocreales: Cordycipitaceae) and one isolate of Metarhizium brunneum (Petch) (ORP-13) (Hypocreales: Clavicipitaceae) against the second instar larvae of the tomato leafminer, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) was determined.
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Key Findings & Data:The study was conducted in 2023 at the Entomology Laboratory, Department of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University.
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Conclusion & Impact:Promising results were obtained in the control of tomato leaf miner using the GOPT-DYYLD and BBE2 isolates.
🔬Design:Araştırma Makalesi
📍Scope:2023 Verileri
⚙️Variables:Beauveria bassiana · entomopatojen fungus · Metarhizium brunneum

Abstract

In this study, the efficacy of two local isolates of the entomopathogenic fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. (GOPT-DYYLD, BBE2) (Hypocreales: Cordycipitaceae) and one isolate of Metarhizium brunneum (Petch) (ORP-13) (Hypocreales: Clavicipitaceae) against the second instar larvae of the tomato leafminer, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) was determined. The study was conducted in 2023 at the Entomology Laboratory, Department of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University. As positive controls, the commercial preparation of B. bassiana strain Bb-1 and 120 g/L Spinetoram were used. The EPF isolates were evaluated in dose-mortality assays at concentrations ranging from 1×10³ to 1×10⁹ conidia/mL-1. The EPF isolates were evaluated using two different methods: Topical application bioassays and foliar spray application bioassays. In topical application bioassays, at a dose of 1×10⁸ conidia/mL-1 on the 5th day, GOPT-DYYLD caused 69.8% mortality, BBE2 45.8%, ORP-13 29.2%, while B. bassiana strain Bb-1 caused 72.9% mortality and 120 g/L Spinetoram caused 96.9% mortality. In foliar spray application bioassays, at a dose of 1×10⁸ conidia/mL-1 on the 5th day, GOPT-DYYLD caused 76.04% mortality, BBE2 54.2%, and ORP-13 24.5%. Promising results were obtained in the control of tomato leaf miner using the GOPT-DYYLD and BBE2 isolates.

Özet

Bu çalışmada entomopatojen fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae)’nın iki yerel izolatı (GOPT-DYYLD, BBE2) ve Metarhizium brunneum (Petch) (Hypocreales: Clavicipitaceae)’ın bir izolatının domates güvesi, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) 2. dönem larvalarına karşı etkinlikleri belirlenmiştir. Çalışma, 2023 yılında Tokat Gaziosmanpaşa Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Entomoloji Laboratuvarında gerçekleştirilmiştir. Pozitif kontrol olarak ticari preparat B. bassiana strain Bb-1 ve 120 g/L Spinetoram kullanılmıştır. EPF izolatları, 1×10³- 1×10⁹ konidia/mL-1 konsantrasyonlarında doz-ölüm denemelerine tabi tutulmuştur. EPF izolatları, topikal uygulama ve yaprağa püskürtme denemeleri olmak üzere iki farklı yöntemle değerlendirilmiştir. Topikal uygulama denemelerinde 1x108 konidi/mL-1 dozunda 5’inci günde GOPT-DYYLD 69.8%, BBE2 45.8%, ORP-13 29.2% oranında ölüme neden olurken B. bassiana strain Bb-1 72.9%, ve 120 G/L Spinetoram 96.9% oranında ölüm oluşturmuştur. Yaprağa püskürtme denemelerinde EPF’lar 1x108 konidi/mL-1 dozunda 5. gün GOPT-DYYLD 75.5%, BBE2 53.2%, ORP-13 24.5% oranında ölüm göstermiştir. GOPT-DYYLD ve BBE2 izolatlarıyla domates güvesi mücadelesinde ümit verici sonuçlar elde edilmiştir.

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Cite This Article

Kara, N., Yanar, D. (2026). Evaluation of the insecticidal activity of some Turkish entomopathogenic fungal isolates against second larval stage of Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae). Türkiye Entomoloji Dergisi, Cilt: 49 Sayı: 4, 401–412. https://doi.org/10.16970/entoted.1700140

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