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Journals / Türkiye Entomoloji Dergisi / 2026 / Cilt: 50 Sayı: 2

First isolation and identification of Serratia marcescens J (Enterobacterales: Yersiniaceae) in a commercial colony of Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae)

Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae)'nın ticari kolonisinde Serratia marcescens J (Enterobacterales: Yersiniaceae)'in ilk izolasyonu ve tanımlanması

Merve Almula Bakırdöğen Bakar(ERZURUM TEKNİK ÜNİVERSİTESİ)0000-0002-7381-473X
Gözde Büşra Eroğlu(ERZURUM TEKNİK ÜNİVERSİTESİ)0000-0001-8988-1315
Pages
137–146
Publication Date
2026-06-26
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings highlight potential mass mortality events in the live feed trade, emphasizing economic losses, zoonotic and pathogenic risks, and the critical importance of hygiene and biosecurity measures.

📌 3 Key Takeaways

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Objective & Hypothesis:The speckled cockroach, Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae), is a common live feed source for reptiles, amphibians, insectivorous birds, and predatory invertebrates due to its high nutritional value.
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Key Findings & Data:On 26 July 2025, adult individuals obtained from a pet shop in Ankara were brought to the laboratory, and shortly thereafter, they exhibited pink feces, signs of decomposition, and mass mortality.
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Conclusion & Impact:The findings highlight potential mass mortality events in the live feed trade, emphasizing economic losses, zoonotic and pathogenic risks, and the critical importance of hygiene and biosecurity measures.
🔬Design:Araştırma Makalesi
📍Scope:2025 Verileri
⚙️Variables:Ticari koloni · entomopatojenik bakteri · böcek mortalitesi

Abstract

The speckled cockroach, Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae), is a common live feed source for reptiles, amphibians, insectivorous birds, and predatory invertebrates due to its high nutritional value. On 26 July 2025, adult individuals obtained from a pet shop in Ankara were brought to the laboratory, and shortly thereafter, they exhibited pink feces, signs of decomposition, and mass mortality. Light microscopy of suspensions prepared from the cadavers revealed bacterial cells, which were subsequently cultured on LB agar. The colonies appeared dark red, and Gram staining indicated that the bacterium was Gram-negative with a bacillus morphology. Blastn analysis of the 16S rRNA gene region of the isolated bacterium confirmed its identity as Serratia marcescens J (Enterobacterales: Yersiniaceae) with 100% similarity. Phylogenetic analysis placed it in the same cluster as another isolate reported from Türkiye (accession number: KX959982.1), supported by a bootstrap value of 80%. Adult female N. cinerea individuals were experimentally infected with five different doses of S. marcescens (1 × 105–1 × 109 cfu/mL). After fourteen days, the highest dose resulted in 80% mortality, and the LD₅₀ was calculated as 9.84 × 105 cfu/mL. This study demonstrates, for the first time, the isolation of S. marcescens from the live feed N. cinerea and its high virulence in these insects. The findings highlight potential mass mortality events in the live feed trade, emphasizing economic losses, zoonotic and pathogenic risks, and the critical importance of hygiene and biosecurity measures.

Özet

Benekli hamam böceği, Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae), yüksek besin değeri nedeniyle sürüngenler, amfibiler, böcekçil kuşlar ve avcı omurgasızlar için yaygın bir canlı yem kaynağıdır. 26 Temmuz 2025’te Ankara’daki bir petshoptan temin edilen ergin bireyler, laboratuvara getirildikten kısa süre sonra pembe dışkı ve erime belirtileri ile toplu olarak ölmüştür. Kadavralardan hazırlanan süspansiyon ile yapılan ışık mikroskobisi analizinde bakteri hücreleri saptanmış ve LB agar üzerinde kültürlenmiştir. Koloniler koyu kırmızı renkte olup, Gram boyama sonuçları bakterinin Gram-negatif ve basil morfolojisine sahip olduğunu göstermiştir. İzole edilen bakterinin 16S rRNA gen bölgesinin Blastn analizi, %100 benzerlik ile Serratia marcescens J (Enterobacterales: Yersiniaceae) olduğunu doğrulamıştır. Filogenetik analizde ise Türkiye’den bildirilen bir diğer izolat (erişim numarası: KX959982.1) ile aynı kümeye yerleşmiş olup, bu ilişki %80 bootstrap değeri ile desteklenmektedir. Ergin dişi N. cinerea bireyleri, S. marcescens’in beş farklı dozu (1 × 105–1 × 109 cfu/mL) ile enfekte edilmiştir. On dört gün sonunda en yüksek dozda ölüm oranı %80 olarak belirlenmiş ve LD₅₀ değeri 9.84 × 105 cfu/mL olarak hesaplanmıştır. Bu çalışma, S. marcescens’in canlı yem N. cinerea’dan ilk kez izole edildiğini ve böceklerde yüksek virülansa sebep olduğunu göstermektedir. Bulgular, canlı yem ticaretinde ekonomik kayıplara yol açabilecek toplu ölümler ile zoonotik ve patojenik riskleri vurgulamakta, hijyen ve biyogüvenlik önlemlerinin önemini ortaya koymaktadır.

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Cite This Article

Bakar, M. A. B., Eroğlu, G. B. (2026). First isolation and identification of Serratia marcescens J (Enterobacterales: Yersiniaceae) in a commercial colony of Nauphoeta cinerea (Olivier, 1789) (Blattodea: Blaberidae). Türkiye Entomoloji Dergisi, Cilt: 50 Sayı: 2, 137–146. https://doi.org/10.16970/entoted.1846834

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References (47)

  1. Abbott, W. S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18 (2): 265-267.
  2. Adedara, I. A., D. B. Rosemberg, D. de Souza, E. O. Farombi, M. Aschner, D. O. Souza & J. B. Rocha, 2016. Neurobehavioral and biochemical changes in Nauphoeta cinerea following dietary exposure to chlorpyrifos. Pesticide Biochemistry and Physiology, 130 (2016): 22-30.
  3. Adedara, I. A., K. A. Mohammed, J. Canzian, D. B. Rosemberg, M. Aschner, E. O. Farombi & J. B. Rocha, 2023. Nauphoeta cinerea as an emerging model in neurotoxicology. In Advances in Neurotoxicology, 9: 181-196.
  4. Bai, Y., J. Henry, O. Campana & D. Wlodkowic, 2021. Emerging prospects of integrated bioanalytical systems in neuro-behavioral toxicology. Science of the Total Environment, 756 (2021): 143922 (1-12).
  5. Bakirdogen, M. A. & G. B. Eroglu, 2024. A highly active Chitinase-A of Serratia ficaria isolated from Pieris brassicae (Lepidoptera: Pieridae). Crop Protection, 179 (2024): 106623 (1-6).
  6. Bouchebti, S., V. Durier, C. Pasquaretta, C. Rivault & M. Lihoreau, 2016. Subsocial cockroaches Nauphoeta cinerea mate indiscriminately with kin despite high costs of inbreeding. PLoS One, 11 (9): e0162548 (1-17).
  7. Brenner, R. J. & R. D. Kramer, 2019. “Cockroaches (Blattaria), 61-77”. In: Medical and Veterinary Entomology (Eds. G. R. Mullen & L. A. Durden). Academic Press, 769 pp.
  8. Bruton, B. D., F. Mitchell, J. Fletcher, S. D. Pair, A. Wayadande, U. Melcher, J. Brady, B. Bextine & T. W. Popham, 2003. Serratia marcescens, a phloem-colonizing, squash bug-transmitted bacterium: causal agent of cucurbit yellow vine disease. Plant Disease, 87 (8): 937-944.
  9. Chaichanawongsaroj, N., K. Vanichayatanarak, T. Pipatkullachat, M. Polrojpanya & S. Somkiatcharoen, 2004. Isolation of gram-negative bacteria from cockroaches trapped from urban environment. Southeast Asian Journal of Tropical Medicine and Public Health, 35 (3): 681-684.
  10. Chitsazi, S., G. H. Moravvej & M. Naderi-Nasab, 2013. A survey on the bacterial and fungal contamination of German cockroaches in Mashhad Imam Reza hospital during 2009-2010. Feyz Journal of Kashan University of Medical Sciences, 16 (6): 576-584.
  11. Cochran, D. G., 2009. “Blattodea: (Cockroaches), 108-112”. In: Encyclopedia of Insects (Eds. V. H. Resh & R. T. Cardé). Academic Press, 1132 pp.
  12. Cotton, M. F., E. Wasserman, C. H. Pieper, D. C. Theron, D. van Tubbergh, G. Campbell, F. C. Fang & A. J. Barnes, 2000. Invasive disease due to extended spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: the possible role of cockroaches. Journal of Hospital Infection, 44 (1): 13-17.
  13. Cutler, J., K. Hughes & R. Rae, 2017. Susceptibility of cockroaches (Gromphadorhina portentosa, Nauphoeta cinerea and Blaptica dubia) exposed to entomopathogenic nematodes. Biocontrol Science and Technology, 27 (4): 556-564.
  14. De Lillo, A., F. P. Ashley, R. M. Palmer, M. A. Munson, L. Kyriacou, A. J. Weightman & W. G. Wade, 2006. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral microbiology and immunology, 21 (1): 61-68.
  15. Eski, A., İ. Demir, M. Güllü & Z. Demirbağ, 2018. Biodiversity and pathogenicity of bacteria associated with the gut microbiota of beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae). Microbial pathogenesis, 121 (2018): 350-358.
  16. Fakoorziba, M. R., F. Eghbal, J. Hassanzadeh & M. D. Moemenbellah-Fard, 2010. Cockroaches (Periplaneta americana and Blattella germanica) as potential vectors of pathogenic bacteria in Shiraz, Iran. Iranian Journal of Medical Sciences, 35 (4): 296-300.
  17. Fodor, A., L. Palkovics & A. Végh, 2022. First report of Serratia marcescens from oleander in Hungary. Phytopathol. Mediterr, 61 (2): 311-317.
  18. Gahukar, R. T., 2016. “Edible Insects Farming: Efficiency and Impact on Family Livelihood, Food Security, and Environment Compared with Livestock and Crops, 85-111”. In: Insects as Sustainable Food Ingredients (Eds. A. T. Dossey, J. A. Morales-Ramos & M. G. Rojas). New York: Academic Press, 385 pp.
  19. Grimont, P. A. & F. Grimont, 1978. The genus Serratia. Annual Reviews in Microbiology, 32 (1): 221-248.
  20. Guiné, R. P., P. Correia, C. Coelho & C. A. Costa, 2021. The role of edible insects to mitigate challenges for sustainability. Open Agriculture, 6 (1): 24-36.
  21. Harris, W. E. & P. J. Moore, 2005. Sperm competition and male ejaculate investment in Nauphoeta cinerea: Effects of social environment during development. Journal of Evolutionary Biology, 18 (2): 474-480.
  22. Holken Lorensi, G., R. Soares Oliveira, A. P. Leal, A. P. Zanatta, C. G. Moreira de Almeida, Y. C. Barreto, M. E. Rosa, P. de Brum Vieira, C. J. B. Ramos, F. de Carvalho Victoria, A. B. Pereira, V. L. Teixeira & C. A. Dal Belo, 2019. Entomotoxic activity of Prasiola crispa (Antarctic algae) in Nauphoeta cinerea cockroaches: identification of Main steroidal compounds. Marine Drugs, 17 (10): 573 (1-13).
  23. Juárez‐Barrientos, J. M., J. Rodríguez‐Miranda, E. S. Peña‐Marín, B. Chareo‐Benítez, J. P. Alcántar‐Vázquez, E. D. J. Ramírez‐Rivera, A. Aparicio-Saguilán & A. G. da Cruz, 2024. Chemical composition, thermal profile and functional properties of grasshopper (Sphenarium purpurascens Ch.), cockroach (Nauphoeta cinerea) flours and their mixtures. International Journal of Food Science & Technology, 59 (8): 5829-5836.
  24. Kang, B., D. Vellody, H. Homburger & J. W. Yunginger, 1979. Cockroach cause of allergic asthma: its specificity and immunologic profile. Journal of Allergy and Clinical Immunology, 63 (2): 80-86.
  25. Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2): 111-120.
  26. Konecka, E., J. Mokracka, S. Krzymińska & A. Kaznowski, 2019. Evaluation of the pathogenic potential of insecticidal Serratia marcescens strains to humans. Polish Journal of Microbiology, 68 (2): 185 (1-7).
  27. Kulma, M., V. Plachý, L. Kouřimská, V. Vrabec, T. Bubová, A. Adámková & B. Hučko, 2016. Nutritional value of three Blattodea species used as feed for animals. Journal of Animal and Feed Sciences, 25 (4): 354-360.
  28. Lanzrein, B., M. Hashimoto, V. Parmakovich, K. Nakanishi, R. Wilhelm & M. Lüscher, 1975. Identification and quantification of juvenile hormones from different developmental stages of the cockroach Nauphoeta cinerea. Life Sciences, 16 (8): 1271-1284.
  29. Li, M., C. Mao, X. Li, L. Jiang, W. Zhang, M. Li, H. Liu, Y. Fang, S. Liu, G. Yang & X. Hou, 2023. Edible insects: A new sustainable nutritional resource worth promoting. Foods, 12 (22): 4073 (1-23).
  30. Molewa, M. L., T. Barnard & N. Naicker, 2022. A potential role of cockroaches in the transmission of pathogenic bacteria with antibiotic resistance: A scoping review. The Journal of Infection in Developing Countries, 16 (11): 1671-1678.
  31. Morton, A., & F. Garcia del Pino, 2013. Sex-related differences in the susceptibility of Periplaneta americana and Capnodis tenebrionis to the entomopathogenic nematode Steinernema carpocapsae. Journal of Invertebrate Pathology, 112 (3): 203–207.
  32. Ng, W. K., K. T. Koay & C. Y. Lee, 2024. Nutrient-enriched live lobster cockroach, Nauphoeta cinerea, enhances growth and pigmentation of the pearl arowana, Scleropages jardini. Journal of Insects as Food and Feed, 11 (3): 581-591.
  33. Oliveira, L. M., A. J. Silva Lucas, C. L. Cadaval & M. S. Mellado, 2017. Bread enriched with flour from cinereous cockroach (Nauphoeta cinerea). Innovative Food Science & Emerging Technologies, 44 (2017): 30–35.
  34. Pai, H. H., W. C. Chen & C. F. Peng, 2004. Cockroaches as potential vectors of nosocomial infections. Infection Control & Hospital Epidemiology, 25 (11): 979-984.
  35. Prado, M. A., E. Gir, M. S. Pereira, C. Reis & F. C. Pimenta, 2006. Profile of antimicrobial resistance of bacteria isolated from cockroaches (Periplaneta americana) in a Brazilian health care institution. Brazilian Journal of Infectious Diseases, 10 (1): 26-32.
  36. Rossato, J. M., T. R. Moresco, J. Uczay & J. B. T. da Rocha, 2019. Staphylococcus aureus-induced sepsis in the lobster cockroach Nauphoeta cinerea. Comparative Immunology, Microbiology and Infectious Diseases, 66 (2019): 101343 (1-8).
  37. Sanchez-Contreras, M. & I. Vlisidou, 2008. The diversity of insect-bacteria interactions and its applications for disease control. Biotechnology and Genetic Engineering Reviews, 25 (1): 203-244.
  38. Sanchez, O., J. Robla, A. Pérez‐Gómez & A. Arias, 2024. First record of the lobster cockroach Nauphoeta cinerea (Olivier, 1789) (Insecta: Blattodea) in Europe with remarks on synanthropic cockroaches of the Iberian Peninsula. Journal of Applied Entomology, 148 (9): 1132-1143.
  39. Sheridan, L. A., R. Poulin, D. F. Ward & M. Zuk, 2000. Sex differences in parasitic infections among arthropod hosts: is there a male bias?. Oikos, 88 (2): 327-334.
  40. Siddiqui, S. A., M. C. I. Hadus, A. Fitriani, V. Guleria, S. Kuppusamy, B. Bhattacharjee, B. Yudhistira & A. Maggiolino, 2024. Edible cockroaches as food and feed–A systematic review on health benefits, nutritional aspects and consumer acceptance. Journal of Insects as Food and Feed, 1 (aop) 12 (2): 179-221.
  41. Tatfeng, Y. M., M. U. Usuanlele, A. Orukpe, A. K. Digban, M. Okodua, F. Oviasogie & A. A. Turay, 2005. Mechanical transmission of pathogenic organisms: the role of cockroaches. Journal of Vector Borne Diseases, 42 (4):129-134.
  42. Tubin, J. S. B., M. G. Sthephaniee, M. del Carmen Monroy-Dosta, M. H. Khanjani & M. G. C. Emerenciano, 2023. Biofloc technology and cockroach (Nauphoeta cinerea) insect meal-based diet for Nile tilapia: zootechnical performance, proximate composition and bacterial profile. Annals of Animal Science, 23 (3): 877-886.
  43. Van Huis, A., J. V. Itterbeeck, H. Klunder, E. Mertens, A. Halloran, G. Muir & P. Vantomme, 2013. Edible Insects: Future Prospects for Food and Feed Security. FAO Forestry Paper 171: 187 pp.
  44. Vazirianzadeh, B., R. Dehghani, M. Mehdinejad, M. Sharififard & N. Nasirabadi, 2014. The first report of drug resistant bacteria isolated from the brown-banded cockroach, Supella longipalpa, in Ahvaz, Southwestern Iran. Journal of Arthropod-Borne Diseases, 8 (1): 53-59.
  45. Veldkamp, T., G. Van Duinkerken & A. Van Huis, 2012. Insects as a sustainable feed ingredient in pig. Food Chemistry, 50: 192-195.
  46. Zhao, H., M. Jiang, M., X. Wang, H. Gao, Y. Zhang, J. Wang, X. Zhang, D. Zhao & F. Zhang, 2024. Pathogenic fungi synergistically cooperate with Serratia marcescens to increase cockroach mortality. Pesticide Biochemistry and Physiology, 202 (2024): 105951 (1-11).
  47. Zurek, L. & C. Schal, 2004. Evaluation of the German cockroach (Blattella germanica) as a vector for bacteria of medical importance. Journal of Medical Entomology, 41 (4): 637-643.