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Journals / Turkish Journal of Medical Sciences / 2018 / Cilt: 48 - Sayı: 6

Interleukin 12B mRNA level and rs3212227 genotyping in peripheral blood mononuclear cells of inflammatory bowel disease patients

Hamid Asadzadeh AGHDAEI
Elnaz EMAMI
Mahyar NOURIAN
Vahid CHALESHI
Peyman TARBAN
Hedieh BALAII
Pedram AZIMZADEH
Shiva IRANI
Shabnam SHAHROKH
Mohammad ROSTAMI-NEJAD
Mohammad Reza ZALI
Pages
1147–1152
Publication Date
2018-12-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our results show that IL-12B expression in IBD may be associated with altered immune and inflammatory responses.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: Inflammatory bowel disease (IBD) is a multifactorial disorder.
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Key Findings & Data:Single nucleotide polymorphisms (SNPs) in the IL-12 gene, which are the main factors to regulate the immune reaction, play an important role in the production of IL-12 molecules.
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Conclusion & Impact:Conclusion: Our results show that IL-12B expression in IBD may be associated with altered immune and inflammatory responses.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Inflammatory bowel disease · interleukin-12 subunit p40 · single nucleotide polymorphisms

Abstract

Background/aim: Inflammatory bowel disease (IBD) is a multifactorial disorder. Single nucleotide polymorphisms (SNPs) in the IL-12 gene, which are the main factors to regulate the immune reaction, play an important role in the production of IL-12 molecules. The aim of this study was to evaluate the correlation between the SNP on position +1188 of the 3 ' UTR region of the IL-12 p40 subunit gene and expression of the IL-12 p40 gene. Materials and methods: This case-control study was performed with 102 patients with IBD and 107 healthy people. PCR-RFLP and comparative real-time PCR were performed to assess the association between genotype and IL-12 gene expression. Results: The frequency of AA, CA, and CC genotypes of this gene at position +1188 was calculated to be 58.8%, 32.4%, and 8.8% in patients and 61.7%, 26.2%, and 12.1% in the control group, respectively, with no significant difference between the two groups (IL- 12B rs3212227: AA (Reference 1), CA (P = 0.407); OR (95% CI) 0.771 (0.418-1.424), CC (P = 0.561); OR (95% CI) 1.313 (0.524-3.292)). Also, the IL-12B mRNA expression level was compared between IBD patients and healthy controls and demonstrated a significant association (R 2 0.136, 95% CI 1.892-3.872, P < 0.0001). Conclusion: Our results show that IL-12B expression in IBD may be associated with altered immune and inflammatory responses.

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Cite This Article

AGHDAEI, H. A., EMAMI, E., NOURIAN, M., CHALESHI, V., TARBAN, P., BALAII, H., AZIMZADEH, P., IRANI, S., SHAHROKH, S., ROSTAMI-NEJAD, M., ZALI, M. R. (2018). Interleukin 12B mRNA level and rs3212227 genotyping in peripheral blood mononuclear cells of inflammatory bowel disease patients. Turkish Journal of Medical Sciences, Cilt: 48 - Sayı: 6, 1147–1152. https://ajindex.com/articles/113845

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