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Journals / Mersin Üniversitesi Sağlık Bilimleri Dergisi / 2023 / Cilt: 16 Sayı: 3

Association study of BDNF gene polymorphism (rs6265) and BDNF gene expression levels in patients with major depression

Majör depresyon hastalarında BDNF gen polimorfizmi (rs6265) ile BDNF gen ekspresyon düzeylerinin araştırılması

Ümit Karakaş(BAYBURT ÜNİVERSİTESİ)0000-0001-9321-5876
Kenan Çevik(MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-2108-1316
Mustafa Ertan Ay(MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-6152-7450
Gurbet Doğru Özdemir0000-0002-6493-3403
Alper Zıblak(Denizli Devlet Hastanesi)0009-0004-2839-837X
Ayşe Nur İnci Kenar(PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-9206-7170
Didem Derici Yıldırım(MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-7709-6133
Mehmet Emin Erdal(MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-6191-2930
Pages
473–482
Publication Date
2023-12-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, in the age of personalized medicine, understanding global and specific factors that can affect the effectiveness of clinical interventions can lead to significant advances in medicine.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: A better understanding of the genetic, molecular, and cellular mechanisms of brain-derived neurotrophic factor (BDNF) and its relationship with neuroplasticity could be pivotal in finding future therapeutic targets for new drugs in major depression (MD).
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Key Findings & Data:This study was planned to understand the relationship between BDNF-related polymorphism (rs6265) and expression in MD.
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Conclusion & Impact:In addition, in the age of personalized medicine, understanding global and specific factors that can affect the effectiveness of clinical interventions can lead to significant advances in medicine.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Beyin kaynaklı nörotrofik faktör · ekspresyon · majör depresyon

Abstract

Aim: A better understanding of the genetic, molecular, and cellular mechanisms of brain-derived neurotrophic factor (BDNF) and its relationship with neuroplasticity could be pivotal in finding future therapeutic targets for new drugs in major depression (MD). This study was planned to understand the relationship between BDNF-related polymorphism (rs6265) and expression in MD. There are still conflicting results regarding the precise role of BDNF polymorphisms in MD. Method: In this case-control study, single nucleotide amplification studies were performed by Real-Time PCR with primers designed for BDNF gene Val66Met (rs6265) polymorphisms from the blood of 51 patients with MD and 75 healthy controls according to DSM-5 criteria. The total RNA obtained was used for gene expression analyses. The relationship between the expression levels of the BDNF gene in the MD and control groups, which were determined by the quantitative Real-Time PCR method, and the polymorphisms were analyzed by statistical methods. Results: No statistically significant correlation was observed between the genotype and the group also between the allele frequency and the group in males and females. It was observed that there was no significant relationship between allele and MD and between genotype and MD. There is no statistically significant difference between genotype groups regarding BDNF expression levels. No difference was observed between the polymorphic genotypes of BDNF in terms of expression levels for the MD and the control groups. Conclusion: Although the study findings were not statistically significant, we think that in the light of previous studies reported, more in-depth analyzes of BDNF variants may point to possible new targets for future pharmaceutical intervention and precision medicine for major depression. In addition, in the age of personalized medicine, understanding global and specific factors that can affect the effectiveness of clinical interventions can lead to significant advances in medicine.

Özet

Amaç: Beyin kaynaklı nörotrofik faktörün (BDNF) genetik, moleküler ve hücresel mekanizmalarının ve bunun nöroplastisite ile ilişkisinin daha iyi anlaşılması, majör depresyonda (MD) yeni ilaçlar için gelecekteki terapötik hedeflerin bulunmasında çok önemli bir rol oynayabilir. Bu çalışma, BDNF polimorfizmlerinin MD'deki kesin rolüne ilişkin hala çelişkili sonuçlar olduğundan, MD'de BDNF ile ilişkili polimorfizm (rs6265) ve ekspresyon ilişkisini anlamak amacıyla planlandı. Yöntem: Bu olgu-kontrol çalışmasında, DSM-5 ölçütlerine göre MD’li 51 hasta ve 75 sağlıklı kontrole ait kanlardan BDNF gen Val66Met (rs6265) polimorfizmleri için dizayn edilmiş primerler ile Real-Time PCR ile tek nükleotid polimorfizm çalışmaları yapıldı. Gen ekspresyon analizleri için total RNA kullanıldı. Kantitatif Real-Time PCR yöntemi ile saptanan, MD ve kontrol gruplarındaki BDNF genine ait ekspresyon düzeyleri ile polimorfizmler arasındaki ilişki istatistiksel yöntemlerle incelendi. Bulgular: Erkek ve kadınlarda, genotip ile grup ve alel frekansı ile grup arasında istatistiksel olarak anlamlı bir ilişki gözlenmemiştir. Alel ile MD arasındaki ilişkide ve genotip ile MD arasında anlamlı bir ilişki olmadığı görülmüştür. Genotip grupları arasında BDNF ekspresyon düzeyleri bakımından istatistiksel olarak anlamlı bir farklılık yoktur. Hem MD grubu hem de kontrol grubu için BDNF’nin polimorfik genotipleri arasında ekspresyon düzeyleri bakımından fark gözlenmemiştir. Sonuç: Çalışma bulguları istatistiksel olarak önemli olmamasına rağmen rapor edilmiş önceki çalışmaların da ışığında BDNF varyantlarının daha derinlemesine analizlerinin, gelecekte farmasötik müdahale ve majör depresyonun hassas tıbbı için olası yeni hedeflere işaret edebileceğini düşünmekteyiz. Ayrıca kişiselleştirilmiş tıp çağında, klinik müdahalelerin etkinliğini etkileyebilecek hem küresel hem de spesifik faktörlerin anlaşılması, tıp alanında önemli ilerlemeler sağlayabilir

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Cite This Article

Karakaş, Ü., Çevik, K., Ay, M. E., Özdemir, G. D., Zıblak, A., Kenar, A. N. İ., Yıldırım, D. D., Erdal, M. E. (2023). Association study of BDNF gene polymorphism (rs6265) and BDNF gene expression levels in patients with major depression. Mersin Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 16 Sayı: 3, 473–482. https://ajindex.com/articles/1109135

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