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Journals / European Journal of Biology / 2026 / Volume: 82 Issue: 1

Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding

Yüksel Gezgin(EGE UNIVERSITY)0000-0001-5812-1882
Gülce Güralp(SABANCI UNIVERSITY)0000-0001-5812-1882
Ayşe Berçin Barlas(DOKUZ EYLUL UNIVERSITY)0000-0003-3100-9553
Rengin Eltem(EGE UNIVERSITY)0000-0002-0642-7676
Pages
—
Publication Date
2023-06-26
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Trichoderma genus are environmentally friendly, targeted biocontrol agents used in organic agriculture.
📊
Key Findings & Data:Materials and Methods: Two different gene regions (the nuclear ribosomal internal transcribed spacer (ITS) and translation elongation factor 1 (EF) were used for molecular identification of Trichoderma isolates in this study.
💡
Conclusion & Impact:Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:T. citrinoviride · T. atroviride · BLAST

Abstract

Objective: Trichoderma genus are environmentally friendly, targeted biocontrol agents used in organic agriculture. Currently, due to the increasing number of organic farming practices, Trichoderma species form a good market as commercial biocontrol agents. This study aims to make morphological and molecular identification of Trichoderma isolates, which were found to be potential biocontrol agents against plant pathogenic fungi, and to perform phylogenetic diversity analyses of these species using different bioinformatics tools. Materials and Methods: Two different gene regions (the nuclear ribosomal internal transcribed spacer (ITS) and translation elongation factor 1 (EF) were used for molecular identification of Trichoderma isolates in this study. Polymerase Chain Reaction (PCR) related regions were amplified and sequenced using primers specific to these gene regions. Following molecular identifications based on these two different gene regions, phylogenetic trees were drawn and polymorphic regions in the nucleotide sequences of these genes were determined. Results: As a result of the study, Trichoderma isolates were determined as T. citrinoviride Bissett and T. atroviride P. Karst. at the species level. This study not only provides essential information about the biodiversity of Trichoderma species, which is a biocontrol agent, but also allows the design of new species-specific primers based on the polymorphic regions of both species. Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.

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Cite This Article

Gezgin, Y., Güralp, G., Barlas, A. B., Eltem, R. (2023). Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology, Volume: 82 Issue: 1. https://doi.org/10.26650/EurJBiol.2023.1279151

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