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Journals / European Journal of Biology / 2025 / Volume: 84 Issue: 2

Antioxidant and Antibacterial Activities of Methanolic, Dichloromethane, and n-Hexane Extracts of Crataegus azarolus Root

Abdolkarim Moazeni-roodi(Iranshahr University of Medical Sciences)0000-0002-3561-0592
Mohammad Hedayat-Moghadam(Iranshahr University of Medical Sciences)0009-0007-1108-0113
Mehdi Kamali-Pour(Iranshahr University of Medical Sciences)0009-0000-0598-2216
Hossein Ali Rahdar(Iranshahr University of Medical Sciences)0000-0003-1583-9936
Maryam Yousefi(Iranshahr University of Medical Sciences)0009-0008-9362-6307
Mohammad Basami(Iranshahr University of Medical Sciences)0009-0002-2076-4318
Seyed Mohammad-Sadegh Mousavi(Iranshahr University of Medical Sciences)0009-0002-3858-2897
Seyed Mohammadreza Emadi(Iranshahr University of Medical Sciences)0009-0000-7183-0691
Pages
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Publication Date
2025-12-18
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: This study determined that methanol solvent is an appropriate choice for effectively extracting the active components from the plant root, as demonstrated by the extraction yield results and the overall phenolic and flavonoid composition assessment.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Crataegus azarolus (yellow hawthorn) is an edible medicinal plant.
📊
Key Findings & Data:Results: This study determined that methanol solvent is an appropriate choice for effectively extracting the active components from the plant root, as demonstrated by the extraction yield results and the overall phenolic and flavonoid composition assessment.
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Conclusion & Impact:Conclusion: The methanolic extract of yellow hawthorn, characterised by a high extraction yield and significant antioxidant and antibacterial properties, is a superior choice for pharmacological study.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Yellow hawthorn · Antioxidant · Antibacterial

Abstract

Objective: Crataegus azarolus (yellow hawthorn) is an edible medicinal plant. This study aims to quan, titatively evaluate the total phenolic and flavonoid compounds in methanolic, dichloromethane, and n,hexane extracts of the roots of this plant and investigate their antioxidant and antibacterial activities. Materials and Methods: The Soxhlet method was used to obtain plant extracts. The Folin,Ciocalteu assay for phenolics and the aluminium chloride assay for flavonoids were used. The Ferric Reducing Antioxidant Power method was used to determine the antioxidant potential and antibacterial properties through agar disc diffusion and broth microdilution methods. Results: This study determined that methanol solvent is an appropriate choice for effectively extracting the active components from the plant root, as demonstrated by the extraction yield results and the overall phenolic and flavonoid composition assessment. A strong and significant relationship between antioxidant activity and phenol content was identified. Compared with other extracts, the methanolic extract showed a high antimicrobial efficacy against Gram,positive and Gram,negative bacteria. Conclusion: The methanolic extract of yellow hawthorn, characterised by a high extraction yield and significant antioxidant and antibacterial properties, is a superior choice for pharmacological study.

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Cite This Article

Moazeni-roodi, A., Hedayat-Moghadam, M., Kamali-Pour, M., Rahdar, H. A., Yousefi, M., Basami, M., Mousavi, S. M., Emadi, S. M. (2025). Antioxidant and Antibacterial Activities of Methanolic, Dichloromethane, and n-Hexane Extracts of Crataegus azarolus Root. European Journal of Biology, Volume: 84 Issue: 2. https://doi.org/10.26650/EurJBiol.2025.1613176

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