Development of Ecological Biodesign Products by Bacterial Biocalcification

Abstract

Biodesign is an interdisciplinary field in which biological processes are combined with many different fields to produce environmentally friendly and economically feasible products. Within the scope of this study, first CaCO3 production potential of Sporosarcina pasteurii has been observed and optimized, and then the capability of hardening of the sand is examined. The optimum CaCl2 concentration for maximized CaCO3 formation was found as 50 mM. The ecological urban element was designed and its mold was produced by 3D printer at lab scale. The Sporosarcina pasteurii was mixed with sand and filled into the mold. The sand was mixed with 50 mM CaCl2 solution every day until hardening is observed. At the end of one week, a sitting element from hardened sand was produced. The CaCO3 formation was observed with XPS analysis. Thus, an interdisciplinary approach was used to produce ecological biodesign products.v