Vibrio Infection and Treatment on the Juvenile Rainbow Trout (Oncorhynchus mykiss) Transferred Seawater

Abstract

The aim of this study is to determinethe antibiotic profile, serotyping and identification of isolated bacteria from disease outbreaks in cultured rainbow trout juveniles transferred to seawater at 8°Cfloating net cages in the Eastern Black Sea Region of Turkey. The kidney and spleen of diseased rainbow trout that showed typical disease symptoms was streakedon the surface of T-TSA (added with 1.5% sodium chloride) and TCBS, and was incubated in the cooled incubator at 20±1°C for 48 hours. To identify of isolatedbacteria was carried out by using slide agglutination, API 20E, PCR and conventional biochemical tests. V. anguillarum isolates were detected serotype O1, andsequenced PCR products were confirmed to be similar to V. anguillarum strain at the rate of 99% (with accession numbers: LK021130) compered in GenBankdatabase. Results of the testing susceptibility to antibiotics showed that V. anguillarum isolates were resistant to sulphamethoxazole, ampicillin, erythromycin andoxytetracyclin, but all strains were found susceptible to oxolinic acid, enrofloxacin and florfenicol.