Dergiler / Gaziosmanpaşa Bilimsel Araştırma Dergisi / 2021 / Cilt: 10 - Sayı: 3
Phytochemical screening and radical scavenging activity of green coffee bean extract: Multiple-protective efficacy against Bisphenol A
- Sayfa
- 256–275
- DOI
- —
Özet
In this study, phytochemical characterization, radical scavenging activity and the protective role of green coffee bean extract (Gcbe) against Bisphenol-A (BpA) were evaluated. In phytochemical analysis total phenolic, flavonoid and tannin contents were investigated and major components were determined by HPLC. The amerolative effect of Gcbe against BpA-induced toxicity were studied in albino mice. For this purpose, mice were separated into six groups which treated with tap-water, BpA and two doses of Gcbe. To determine BpA toxicity and the protective role of Gcbe, changes in some biochemical parameters, micronucleus (MN) frequency and DNA fragmentation were investigated. As a result, the phenolic compounds in the Gcbe content were more intense than the other components. In BpA-treated group, serum parameters increased and the antioxidant/oxidant dynamics were impaired. Gcbe treatment decreased the induced serum parameters and caused an improvement in antioxidant-oxidant dynamics and also a decrease in the DNA-tail percentage and MN frequency.
Abstract
In this study, phytochemical characterization, radical scavenging activity and the protective role of green coffee bean extract (Gcbe) against Bisphenol-A (BpA) were evaluated. In phytochemical analysis total phenolic, flavonoid and tannin contents were investigated and major components were determined by HPLC. The amerolative effect of Gcbe against BpA-induced toxicity were studied in albino mice. For this purpose, mice were separated into six groups which treated with tap-water, BpA and two doses of Gcbe. To determine BpA toxicity and the protective role of Gcbe, changes in some biochemical parameters, micronucleus (MN) frequency and DNA fragmentation were investigated. As a result, the phenolic compounds in the Gcbe content were more intense than the other components. In BpA-treated group, serum parameters increased and the antioxidant/oxidant dynamics were impaired. Gcbe treatment decreased the induced serum parameters and caused an improvement in antioxidant-oxidant dynamics and also a decrease in the DNA-tail percentage and MN frequency. Bisphenol A, Comet, Green coffee bean extract, Phytochemical characterization, Radical scavenging activity