Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

Abstract

In this study, A standart protocol to callus formation and adventitious shoot regeneration of Hypericum adenotrichum Spach via direct or indirect organogenesis has been described.Callus induction was carried out by using leaves which were collected from their native environment. The maximum callus induction frequency has been observed on MS media containing 4 mgL-1 BA and 0.2 mgL-1 NAA. These calli were not induced to shoot regeneration. Shoot formation was obtained by transferring the calluses from BA-containing media to MS medium containing 0.5 mgL-1 KIN. Maximum shoot number was observedin transferred calluses from callus induction medium containing 3 mgL-1 BA to media containing 0.5 mgL-1 KIN.Direct shoot formation developed on leaf explants of H. adenotrichum on MS media containing KIN alone. The highest direct shoot development was observed on MS medium with 1 mgL-1 KIN.