Dergiler / International Journal of Secondary Metabolite / 2021 / Cilt: 8 - Sayı: 4
An Investigation of The Biological Activity of Monofloral Honey Produced in South-Western Anatolia
- Sayfa
- 300–311
- DOI
- —
Özet
In this study, monofloral honeys (chaste, thyme, citrus, and heather) which were obtained from different sources from members of Beekeeping Associations in South-West Anatolia were studied for their antioxidant capacity, total phenolic amounts, total flavonoid amounts, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and urease inhibition activities. Antioxidant capacity of honey samples was determined by β-carotene, DPPH, ABTS+ and CUPRAC activity methods. In honey samples, the highest antioxidant activity was found in citrus honey with β-carotene/Linoleic acid color opening with IC50: 7.99 mg/mL, and DPPH free radical removal activity with IC50: 5.28 mg/mL in thyme honey. In CUPRAC activity, it was determined that the highest activity was found in heather honey with IC50: 1.69 mg/mL, in terms of ABTS+ removal activity IC50: 2.80 mg/mL in chaste honey, and metal chelating activity IC50: 1.56 mg/mL in thyme honey. The total phenolic and flavonoids amounts of honeys ranged from 2.31 and 27.15 (μg PEs/mg) to 4.95 and 25.24 (µg QEs/mg), respectively. In addition, AChE inhibition IC50: 24.25 mg/mL in thyme honey, BChE inhibition IC50: 27.93 mg/mL in thyme honey, and urease inhibition IC50: 34.89 mg/mL with citrus honey were determined concerning the highest activity, consecutively.
Abstract
In this study, monofloral honeys (chaste, thyme, citrus, and heather) which were obtained from different sources from members of Beekeeping Associations in South-West Anatolia were studied for their antioxidant capacity, total phenolic amounts, total flavonoid amounts, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and urease inhibition activities. Antioxidant capacity of honey samples was determined by β-carotene, DPPH, ABTS+ and CUPRAC activity methods. In honey samples, the highest antioxidant activity was found in citrus honey with β-carotene/Linoleic acid color opening with IC50: 7.99 mg/mL, and DPPH free radical removal activity with IC50: 5.28 mg/mL in thyme honey. In CUPRAC activity, it was determined that the highest activity was found in heather honey with IC50: 1.69 mg/mL, in terms of ABTS+ removal activity IC50: 2.80 mg/mL in chaste honey, and metal chelating activity IC50: 1.56 mg/mL in thyme honey. The total phenolic and flavonoids amounts of honeys ranged from 2.31 and 27.15 (μg PEs/mg) to 4.95 and 25.24 (µg QEs/mg), respectively. In addition, AChE inhibition IC50: 24.25 mg/mL in thyme honey, BChE inhibition IC50: 27.93 mg/mL in thyme honey, and urease inhibition IC50: 34.89 mg/mL with citrus honey were determined concerning the highest activity, consecutively.