Bacillus sp. ZBP10 SUŞU ile DERİN KÜLTÜR FERMANTASYONUNDA AMİLAZ ÜRETİMİ

Ayşe AVCI, Dzhanser ADEM, İsmail İNAN, Samet YÜKSEL, Kübra Gizem ŞAHİN, Zekeriya KALKAN

Özet

Abstract

Bacillussp. ZBP10 is an amylase producing strain isolated from a soil sample collected from a potatocultivation field in Sakarya. In this study, culture conditions were optimized for Bacillus sp. ZBP10 inorder to increase amylase production using submerged fermentation. The effects of temperature(30-40°C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat,rice and corn starches) and substrate concentration (5-30 g/L) on the production of amylase weredetermined. According to the results, the bacterium produced maximum amount of amylase, when theinitial pH of the medium was 7.0, at 33°C, and within 48 h. Soluble starch was the best substrate amongthe starches tested. Optimum substrate concentration was 20 g/L for enzyme production with which3.57±0.19 U/mL enzymatic activity was obtained