Study on DNA Binding Properties of Nickle (II) Phthalocyanine Compound with 2-Isopropyl-5- Methylphenoxy Substituents.

Abstract

I n this study, previously synthesized Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni (II) phthalocyanine compound. The DNA binding activities of Ni (II) phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni (II) phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding