Investigation of GLUT1, HIF1α and TBX21 Gene Polymorphisms in Laryngeal Cancer

Abstract

Objective: We aimed to investigate the associationof the frequency of three single-nucleotide polymorphisms [glucose transporter isoform 1 (GLUT1) reference single-nucleotide polymorphism (rs) 710218,hypoxia-inducible factor 1 alpha (HIF1α) rs11549465,and T-box transcription factor protein 21 (TBX21)rs17250932], which have been proved to be relatedwith various benign and malignant diseases, with thedevelopment of laryngeal cancer and its size and grade.Methods: In this study, we included 35 patients withlaryngeal cancer and 35 volunteers at least 30 yearsold who had smoked for at least 20 years. DNA wasobtained from the blood samples of the participantsusing an isolation kit. Then, polymorphisms for boththe groups were determined using real-time polymerase chain reaction.Results: No significant differences were detected regarding the genotype and allele frequencies inthe three polymorphisms assessed between the twogroups. In the patient group, on examining the association of polymorphisms with tumor size and grade,no significant relation was observed in three polymorphisms regarding the related parameters.Conclusion: GLUT1, HIF1α, and TBX21 polymorphisms have no impact on the development of laryngeal cancer.