The association of methylene tetrahydrofolate reductase (MTHFR) A1298C gene polymorphism, homocysteine, vitamin B12, and folate with coronary artery disease (CAD) in the north of Iran

Abstract

Background: We pursued to find out the possible associationof Methylene tetrahydrofolate reductase (MTHFR)A1298C gene polymorphism, blood homocysteine, vitaminB12, and folate with Coronary artery disease (CAD) in thestudy population in Guilan, north of Iran.Material and Methods: Ninety patients with CAD and 76healthy controls were evaluated. MTHFR A1298C polymorphismand its genotype frequency, the plasma level ofhomocysteine, vitamin B12 and folate were evaluated byusing ARMS-PCR, ELISA, and Chemiluminescencemethods, respectively.Results: The frequency of genotypes, A, AC and CC in CADwere 40, 35.6, 24.4%, respectively which was significantlydifferent (p=0.016) from the control group that were 26.3,57.9 and 15.8%, respectively. The serum level of vitaminB12 and folate in genotype A1298C were not statisticallysignificant between two groups (p>0.05), however, theplasma homocysteine in patients with CAD wasremarkably higher than the control group (p