An investigation of antibacterial effects of steroids

Abstract

In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterolon some bacteria were investigated. In the research, standard strains of the gram-positive bacteriaBacillus subtilis(lab isolate) andStaphylococcus aureus(ATCC 6538) and the gram-negative bacteriaEscherichia coli(ATCC 25922),Pasteurella multocida(ATCC 12945),andPseudomonas aeruginosa(ATCC 9027) were used. A modified broth dilution method was applied for determining the minimalinhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions andas a medium. At the end of the study, the MIC of corticosterone forP. multocidaand the MIC of beta-sitosterol forS. aureuswere bothdetermined as 32 μg/mL. However, other steroids showed no effects on the bacteria. These steroids did not appear to have widespreadantibacterial properties.