Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

Abstract

The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)- producingEscherichia coliin healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broilerslaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified usinga BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypicconfirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed withXbaI for the clonal typing of ESBL-producingE. coliisolates. While 33 phenotypic ESBL-producingE. coliisolates were identified, eightof them had only the blaTEM-1. Twenty-five ESBL-producing isolates were detected. This research is the first on the investigation anddetection of ESBL-producingE. coliisolates from broilers in Turkey. In this study, 8.3% ESBL-producingE. coliwere isolated from thecloacal samples of broilers collected from slaughterhouses in Turkey. CTX-M-15 (80%) was the most frequently isolated ESBL type.Using PFGE analysis, it was determined that these isolates had clonal similarity.