Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue

Abstract

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adiposetissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purposeof this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certainsurface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cellsfrom adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. Thecells were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Stem cells were characterized by immunostaining ofCD90, CD105, CD11b, and CD45 surface markers. ADSC growth kinetics were determined at the first, second, and third passages. Inconclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and populationdoubling times during the first, second, and third passages.