Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers

Abstract

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogeneticrelationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR),East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samplesusing an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypeswere determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) meanallele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however,only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general FIS values werebetween 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observedgenetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical originsof Turkish native cattle breeds.