Investigation of the effect of combined use of alloplastic-based tricalcium phosphate bone graft and antihemorrhagic plant extract (ABS) on bone regeneration in surgically induced bone defects in nondiabetic rats: an experimental animal study

Abstract

Background/aim: In this experimental study, we aimed to evaluate the late period effects of the combination of Ankaferd BloodStopper (ABS), which has bone wound healing effects, and β-tricalcium phosphate (TCP) on the regeneration of bone tissue throughhistopathological, immunohistochemical, and radiological (dual energy X-ray absorptiometry - DEXA) methods in nondiabetic rats.Materials and methods: Sixty-four Wistar albino male rats were used. In the calvaria of the rats, a bone defect 7.0 mm in diameter wascreated. These rats were divided into 4 different groups. Group 1 was the control group without any treatment, a 0.125 mL Β-TCP graftwas applied to Group 2, a 0.125 mL ABS was applied to Group 3, and a 0.125 mL (β-TCP + ABS) mixture was applied to group 4. Halfof the rats were sacrificed on day 28 and the other half on day 56. Histopathological, immunohistochemical, and DEXA analyses of thespecimens were performed after the experiment.Results: As a result of the histopathological analysis, osteoblastic activity and new bone formation were found to be significantly higherin Group 2, Group 3, and Group 4 than the control group on day 28 (P 0.05).Conclusion: The use of both β-TCP + ABS and only ABS had positive effects on wound healing and bone formation in nondiabetic rats.