Analysing the blood-stemming effect of Ankaferd Blood Stopper in medulla spinalis surgery

Abstract

Background/aim: The aim of this study was to investigate the possible toxicity of the Ankaferd Blood Stopper (ABS) on the neuralsystem.Materials and methods: Thirty Sprague Dawley rats were randomized into ABS (n: 15) and control (n: 15) groups. Following theanaesthetic induction, total laminectomy was performed to the lower thoracic, and upper lumbar areas in both groups and medullaspinalis was exposed. Two myelotomies were performed on the medulla spinalis. One millilitre ABS was applied to the incision site inthe ABS group, and one millilitre 0.9% saline solution was applied in the control group. Rats were observed for 15 days regarding generalbehaviour, neurological signs, mobility, and signs of infection. Sixteen days later, all rats were decapitated under anaesthesia. Medullaspinalis was removed en bloc from all rats and was stained with Heamatoxylin & Eosin and luxol fast blue.Results: There was no significant difference between the ABS group and the control group regarding oedema, gliosis, the intensity ofinflammatory cells, the presence of neuronal degeneration, neuron counts, and myelin degeneration.Conclusion: No clinical or histopathological evidence for the neurotoxic effect of the ABS was observed in the present study. Ourfindings might precipitate the use of ABS on human subjects regarding medulla spinalis surgery.