A novel core-shell-based chromatographic method supported by ratio derivative spectrophotometry for the simultaneous determination of perindopril, indapamide, and amlodipine ternary mixtures

Abstract

In this work, ratio spectra of the first derivative spectrophotometric and liquid chromatographic methodshave been described for the first time for the simultaneous determination of perindopril (PER), indapamide (IND), andamlodipine (AML) in dosage forms. For chromatographic separations several mobile phase compositions were testedfor efficient separation with the use of a new column technology related to superficially porous particles. Optimumchromatographic separation was achieved using a Kinetex C18 column (150 × 4.6 mm I.D. 5 µm) at a flow rate of1.5 mL $min^{-1}$. The separation was carried out at 30 ◦ C and the diode array detector was adjusted to 215 nm. As acomparison, a spectrophotometric method depending on the first derivative of the ratio spectra was developed. The firstderivative of the ratio amplitudes at 227.2 nm for PER, 269.4 nm for AML, 292.0 nm for IND were selected. The proposedmethods were successfully applied for the simultaneous assay of the drug combination in pharmaceutical dosage formsand the methods were compared to each other in terms of Student t and F tests for the comparison of their accuracyand precision parameters.