Synthesis of novel 5-substituted phenyl-3-(p-isopropylphenyl)-1-phenylformazan and their biological activities

Abstract

Novel 1-substituted 3-(p-isopropylphenyl)-5-phenylformazans ( 3a { g ) were synthesized and characterized by elemental analysis, 1 H NMR, and FT-IR techniques and UV-visible spectroscopy. Antiproliferative activities of 3a { g against HeLa and C6 cells were determined using the BrdU cell proliferation ELISA assay. 5-Florouracil was used as the positive control. The effects of substituents ({H, {CH 3 , and {I) and their positions ( ortho , meta , and para ) on the antiproliferative activities were evaluated. The results of the assay indicate that I substituent exhibited higher activity against the cells at the meta and para positions than {CH 3 and {H substituents. 3a { g exhibited both high antiproliferative activities against C6 cells and noncytotoxicity. 3a { g may be anticancer drug candidates.