Sensitive determination of hydrogen peroxide in real water samples by high spin peroxo complex

Abstract

In this paper, a fast, cheap, simple, sensitive and selective spectrophotometric method based on high spinperoxo-Fe(III)-EDTA complex in the alkaline medium was developed for the determination of hydrogen peroxide (H2 O2)in real water samples. The purple-coloured complex with a maximum absorbance at a wavelength of 525 nm wasformed. Various parameters such as type of stabilizer reagent and its concentration, reaction time, Fe(III), EDTA andNH3 concentration were optimized. The method was confirmed with the Beer’s law with a molar absorption coefficientof 267.36 L mol −1cm −1in the 8.3 ×10 −6–4.08 ×10 −3 mol/L concentration range. Sandell’s sensitivity of the proposedmethod was also calculated as 0.188 µg/cm2. LOD and LOQ were determined as 2.5 ×10 −6and 8.3 ×10 −6 mol/L,respectively. Intraday and interday relative standard deviation of the proposed method for 2.0 ×10 −4 mol / L of H2 O2were found as 1.5% and 6.1%, respectively. The developed method is suitable for fast monitoring of H2 O2 in differenttypes of aqueous water samples without any sample preparation steps and acceptable recovery values between 90% and118% were obtained. In the sample analysis, H2 O2 removed solutions from the real water samples were used for blankcorrection in their analysis and this process provides more reliable and accurate results in real sample analysis.