Dergiler / Turkish Journal of Botany / 1997 / Cilt: 21 - Sayı: 1
Susam (Sesamum indicum L.) bitkisinin in-vitro kosullarda rejenerasyonu
- Sayfa
- 15–18
- DOI
- —
Özet
Effects of various plant growth regulators on organogenesis from cotyledon explants of Sesamum indicum L. Muganli-57 were investigated. Murashige and Skoog medium supplemented with 1, 2, 4, 8 mg/l BAP and 0.1 mg/l NAA or without NAA was tested. Callus formation started in ten days and a high frequency of callus formation (up to 100%) was obtained. Adventitious shoots were obtained in an MS medium containing 4 mg/l BAP and 0.1 mg/l NAA or 8 mg/l BAP and 0.1 mg/l NAA after 65 days in culture. Organogenesis occurred at the cut end of the cotyledonary explants. This regeneration system can be used for Agrobacterium-mediated transformation of sesame.
Abstract
Effects of various plant growth regulators on organogenesis from cotyledon explants of Sesamum indicum L. Muganli-57 were investigated. Murashige and Skoog medium supplemented with 1, 2, 4, 8 mg/l BAP and 0.1 mg/l NAA or without NAA was tested. Callus formation started in ten days and a high frequency of callus formation (up to 100%) was obtained. Adventitious shoots were obtained in an MS medium containing 4 mg/l BAP and 0.1 mg/l NAA or 8 mg/l BAP and 0.1 mg/l NAA after 65 days in culture. Organogenesis occurred at the cut end of the cotyledonary explants. This regeneration system can be used for Agrobacterium-mediated transformation of sesame.