Genetic diversity among nile delta isolates of Rhizoctonia solani Kühn based on pathogenicity, compatibility, ısozyme analysis and total protein pattern

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Abstract

The present study obtained 12 isolates from Rhizoctonia solani Kühn isolated from Cotton L, Trifolium L. and Vida faba L. from different localities in the Nile Delta of Egypt. All strains were pathogenic and caused seed rot, wilt, stunting, and preemergence and post-emergence damping-off. The isolated strains produced different forms of infection cushions that ensure the pathogenicity of these strains. SDS-PAGE of the 12 ft solani isolates showed that although the ft solani isolates were isolated from very divergent host plants, the total soluble protein patterns among them were similar. Isozyme analysis revealed higher polymorphism among ft solani isolates using the EST isozyme. The cluster analysis based on isozyme data showed that the ft solani isolates fell into 4 distinct groups, whereas the dendrogram resulting from cluster analysis based on the compatibility test and other morphological characteristics of pathogen infection cushion showed that all ft solani isolates were placed into 3 main distinct groups.