Variation of callus induction through anther culture in water chestnut (Tapa sp.)

Abstract

Abstract: Effects of culture media and genotypes of water chestnut {Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/1 BA, 0.5 mg/1 NAA and 0.1 mg/1 GA3 were suitable for callus induction. Callus yielded poorly in MS-containing media compared to that of N6 media. No callus was induced on 2, 4-D containing media. Among the tested 18 genotypes, only 15 genotypes produced calli.